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首页> 外文期刊>Biochimica et biophysica acta: BBA: International journal of biochemistry, biophysics and molecular biololgy. Proteins and Proteomics >Crystal structure of the C-terminal domain of Mu phage central spike and functions of bound calcium ion
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Crystal structure of the C-terminal domain of Mu phage central spike and functions of bound calcium ion

机译：Mu噬菌体中央突峰C端结构域的晶体结构和结合钙离子的功能

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摘要

Bacteriophage Mu, which has a contractile tail, is one of the most famous genus of Myoviridae. It has a wide host range and is thought to contribute to horizontal gene transfer. The Myoviridae infection process is initiated by adhesion to the host surface. The phage then penetrates the host cell membrane using its tail to inject its genetic material into the host. In this penetration process, Myoviridae phages are proposed to puncture the membrane of the host cell using a central spike located beneath its baseplate. The central spike of the Mu phage is thought to be composed of gene 45 product (gp45), which has a significant sequence homology with the central spike of P2 phage (gpV). We determined the crystal structure of shortened Mu gp45??1-91 (Arg92-Gln197) at 1.5 ? resolution and showed that Mu gp45 is a needlelike structure that punctures the membrane. The apex of Mu gp45 and that of P2 gpV contained iron, chloride, and calcium ions. Although the C-terminal domain of Mu gp45 was sufficient for binding to the E. coli membrane, a mutant D188A, in which the Asp amino acid residue that coordinates the calcium ion was replaced by Ala, did not exhibit a propensity to bind to the membrane. Therefore, we concluded that calcium ion played an important role in interaction with the host cell membrane. ? 2012 Elsevier B.V. All rights reserved.
机译：噬菌体Mu具有尾巴收缩性，是Myoviridae最著名的属之一。它具有广泛的宿主范围，被认为有助于水平基因转移。肌病毒科感染过程是通过粘附于宿主表面而引发的。然后噬菌体使用其尾巴穿透宿主细胞膜，将其遗传物质注入宿主。在这种穿透过程中，提出了Myoviridae噬菌体使用位于其底板下方的中心尖刺穿刺宿主细胞膜的方法。人们认为Mu噬菌体的中央尖峰由基因45产物（gp45）组成，该基因与P2噬菌体（gpV）的中央尖峰具有显着的序列同源性。我们确定了缩短的Mugp45Δε1-91（Arg92-Gln197）的晶体结构为1.5？。分辨率并显示Mu gp45是一种刺穿膜的针状结构。 Mu gp45的顶点和P2 gpV的顶点包含铁，氯和钙离子。尽管Mu gp45的C末端结构域足以结合大肠杆菌膜，但突变体D188A（其中协调钙离子的Asp氨基酸残基被Ala取代）没有表现出与DNA结合的倾向。膜。因此，我们得出结论，钙离子在与宿主细胞膜的相互作用中起着重要作用。 ？ 2012 Elsevier B.V.保留所有权利。

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