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首页> 外文期刊>American Journal of Physiology >Regulation of intestinal NaPi-IIb cotransporter gene expression by estrogen.
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Regulation of intestinal NaPi-IIb cotransporter gene expression by estrogen.

机译：雌激素调节肠道NaPi-IIb共转运蛋白基因表达。

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摘要

The current experiments were designed to study the effect of beta-estradiol on type IIb sodium-coupled phosphate (NaPi-IIb) cotransporter gene expression. Uptake studies with intestinal brush-border membrane vesicles (BBMV) showed that estrogen treatment increased sodium-dependent phosphate absorption by approximately 45% in rat intestine. Northern blot analysis indicated that NaPi-IIb mRNA expression was increased by approximately 50% after estrogen treatment. Western blot analysis also detected an increase in BBMV NaPi-IIb protein expression in estrogen-treated rats. In human intestinal Caco-2 cells, NaPi-IIb mRNA abundance was increased approximately 60% after estrogen treatment, and this increase could be abolished by inhibition of gene transcription. Transfection studies with human NaPi-IIb promoter reporter constructs showed that the promoter was responsive to estrogen treatment. These studies demonstrate for the first time that estrogen stimulates intestinal sodium-dependent phosphate absorptionin female rats. This stimulation is associated with increased NaPi-IIb mRNA and protein expression. Thus the effect of estrogen on intestinal Pi absorption may be partially due to activation of NaPi-IIb gene transcription.
机译：当前的实验旨在研究β-雌二醇对IIb型钠耦合磷酸（NaPi-IIb）共转运蛋白基因表达的影响。肠道刷状边界膜囊泡（BBMV）的摄取研究表明，雌激素治疗可使大鼠肠中钠依赖性磷酸盐的吸收增加约45％。 Northern印迹分析表明，在雌激素处理后，NaPi-IIb mRNA表达增加了约50％。 Western印迹分析还检测到雌激素治疗大鼠中BBMV NaPi-IIb蛋白表达增加。在人肠道Caco-2细胞中，雌激素处理后NaPi-IIb mRNA的丰度增加了大约60％，并且这种增加可以通过抑制基因转录而消除。用人NaPi-IIb启动子报告基因构建体进行转染研究表明，该启动子对雌激素治疗有反应。这些研究首次证明雌激素刺激雌性大鼠肠道钠依赖性磷酸盐的吸收。这种刺激与NaPi-IIb mRNA和蛋白质表达的增加有关。因此，雌激素对肠道Pi吸收的影响可能部分是由于NaPi-IIb基因转录的激活。

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