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首页> 外文期刊>American Journal of Physiology >Influence of acclimation temperature on mitochondrial DNA, RNA, and enzymes in skeletal muscle.
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Influence of acclimation temperature on mitochondrial DNA, RNA, and enzymes in skeletal muscle.

机译：适应温度对骨骼肌线粒体DNA，RNA和酶的影响。

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摘要

Skeletal muscle fibers typically undergo modifications in their mitochondrial content, concomitant with alterations in oxidative metabolism that occur during the development of muscle fiber and in response to physiological stimuli. We examined how cold acclimation affects the mitochondrial properties of two fish skeletal muscle fiber types and how the regulators of mitochondrial content differed between tissues. After 2 mo of acclimation to either 4 or 18 degrees C, mitochondrial enzyme activities in both red and white muscle were higher in cold-acclimated fish. No significant differences were detected between acclimation temperatures in the abundance of steady-state mitochondrial mRNA (cytochrome-c oxidase 1, subunit 6 of F0F1-ATPase), rRNA (16S), or DNA copy number. Steady-state mRNA for nuclear-encoded respiratory (adenine nucleotide translocase 1) and glycolytic genes showed high interindividual variability, particularly in the cold-acclimated fish. Although mitochondrial enzymes were 10-fold different between the two muscle types, mitochondrial DNA copy number differed only 4-fold. The relative abundance of mitochondrial mRNA and nuclear mRNA in red and white muscle reflected the differences in copy number of their respective genes. These data suggest that the response to physiological stimuli and determination of tissue-specific mitochondrial properties likely result from the regulation of nuclear-encoded genes.
机译：骨骼肌纤维的线粒体含量通常会发生变化，伴随着在肌肉纤维发育过程中发生的氧化代谢变化以及对生理刺激的响应。我们研究了冷驯化如何影响两种鱼类骨骼肌纤维类型的线粒体特性，以及组织之间线粒体含量的调节剂如何不同。适应2个月至4或18摄氏度后，冷适应鱼的红色和白色肌肉中的线粒体酶活性更高。在丰富的稳态线粒体mRNA（细胞色素C氧化酶1，F0F1-ATPase的亚基6），rRNA（16S）或DNA拷贝数之间的适应温度之间未检测到显着差异。核编码呼吸道（腺嘌呤核苷酸转位酶1）和糖酵解基因的稳态mRNA显示出很高的个体差异，尤其是在冷适应鱼中。尽管两种肌肉类型之间的线粒体酶相差10倍，但线粒体DNA的拷贝数仅相差4倍。红色和白色肌肉中线粒体mRNA和核mRNA的相对丰度反映了它们各自基因的拷贝数差异。这些数据表明，对生理刺激的反应和组织特异性线粒体特性的确定可能是由于核编码基因的调控所致。

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