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首页> 外文期刊>American Journal of Physiology >Role of actin in EGF-induced alterations in enterocyte SGLT1 expression.
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Role of actin in EGF-induced alterations in enterocyte SGLT1 expression.

机译：肌动蛋白在EGF诱导的肠细胞SGLT1表达改变中的作用。

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摘要

Na+-glucose cotransporter (SGLT1) expression and the role of actin in epidermal growth factor (EGF)-induced alterations in glucose transport and brush-border surface area were examined in New Zealand White rabbit jejunal loops. In separate experiments, EGF or EGF concurrent with cytochalasin D, an inhibitor of actin polymerization, was administered to the experimental loop and compared with its vehicle control. SGLT1 expression was measured by Western blot in brush-border membrane vesicles (BBMV) after 5-min and 1-h exposure. Glucose kinetics were determined by a rapid filtration technique, and brush-border surface area was examined by electron microscopy after 1-h exposure. The effect of cytochalasin D alone on BBMV glucose kinetics and brush-border surface area was also assessed. EGF resulted in a significant increase in BBMV SGLT1 expression (P < 0.05), glucose maximal uptake (Vmax; P < 0.001), and absorptive brush-border surface area (P < 0.001). These effects were abolished with concurrent cytochalasin D treatment. Cytochalasin D alone had no effect on glucose transport or brush-border surface area. The findings suggest that EGF acutely upregulates jejunal brush-border surface area and the Vmax for jejunal glucose uptake via the recruitment and insertion of SGLT1 from an internal pool into the brush border by a mechanism that is dependent on actin polymerization.
机译：在新西兰白兔空肠环路中检查了Na +-葡萄糖共转运蛋白（SGLT1）的表达以及肌动蛋白在表皮生长因子（EGF）诱导的葡萄糖转运和刷状边界表面积变化中的作用。在单独的实验中，将EGF或EGF与肌动蛋白聚合的抑制剂细胞松弛素D并用，将其与实验载体进行比较。暴露5分钟和1小时后，通过Western blot检测刷状边界膜囊泡（BBMV）中的SGLT1表达。通过快速过滤技术测定葡萄糖动力学，并且在暴露1小时后通过电子显微镜检查刷边界表面积。还评估了单独的细胞松弛素D对BBMV葡萄糖动力学和刷边界表面积的影响。 EGF导致BBMV SGLT1表达（P <0.05），葡萄糖最大摄取（Vmax; P <0.001）和吸收性刷毛边界表面积（P <0.001）显着增加。并发细胞松弛素D治疗消除了这些作用。单独的细胞松弛素D对葡萄糖转运或刷状边界表面积没有影响。这些发现表明，EGF通过依赖于肌动蛋白聚合的机制将SGLT1从内部池募集并插入到刷缘中，从而急性上调了空肠刷缘表面积和空肠葡萄糖摄取的Vmax。

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