Comparison of phbC genes cloned from Ralstonia eutropha and Alcaligenes latus for utilization in metabolic engineeringof polyhydroxyalkanoate biosynthesis

Hyun-Dong Shin; Dong-Hyun Oh; Young-Mi Jung; Yong-Hyun Lee; Sa-Youl Ghim

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Comparison of phbC genes cloned from Ralstonia eutropha and Alcaligenes latus for utilization in metabolic engineeringof polyhydroxyalkanoate biosynthesis

机译：从富营养小球藻和产碱杆菌克隆的phbC基因在多羟基链烷酸酯生物合成代谢工程中的应用比较

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摘要

Two cloned phbC genes, encoding polyhydroxybutyrate (PHB) synthase from Ralstonia eutropha and from Alcaligenes latus, were transformed into a PHB-negative mutant of R. eutropha. The expression characteristics of both genes were compared for the biosyntheses of PHB and its copolymers. Each phbC gene had different characteristics not only in the biosyntheses of PHB, poly(3-hydroxybutyrate-3-hydroxy-valerate), and poly(3-hydroxybutyrate-4-hydroxybutyrate) but also in the resulting morphology of PHB granules.

机译：将两个克隆的phbC基因转化为富营养芽孢杆菌的PHB阴性突变体，该基因编码来自富营养小球藻和产自产碱杆菌的多羟基丁酸（PHB）合酶。比较了这两个基因的表达特征，以进行PHB及其共聚物的生物合成。每个phbC基因不仅在PHB，聚（3-羟基丁酸酯-3-羟基-戊酸酯）和聚（3-羟基丁酸酯-4-羟基丁酸酯）的生物合成中具有不同的特征，而且在所得的PHB颗粒形态上也具有不同的特征。

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来源
《Biotechnology Letters》 |2002年第7期|共7页
作者
Hyun-Dong Shin; Dong-Hyun Oh; Young-Mi Jung; Yong-Hyun Lee; Sa-Youl Ghim;
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正文语种 eng
中图分类生物工程学（生物技术）;生命科学总论;分子生物学;
关键词
alcaligenes latus; metabolic engineering; phbC gene; polhydroxy-alkanoates; ralstonia eutropha;

机译：产碱杆菌;代谢工程;phbC基因;多羟基链烷酸酯;富营养度;

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专利

1. Comparison of phbC genes cloned from Ralstonia eutropha and Alcaligenes latus for utilization in metabolic engineeringof polyhydroxyalkanoate biosynthesis [J] . Hyun-Dong Shin, Dong-Hyun Oh, Young-Mi Jung, Biotechnology Letters . 2002,第7期

机译：从富营养小球藻和产碱杆菌克隆的phbC基因在多羟基链烷酸酯生物合成代谢工程中的应用比较
2. Construction of transformant Alcaligenes latus enforcing its own cloned phbC gene and characterization of poly-#beta#-hydroxybutyrate biosynthesis [J] . Lee Yong-Hyun, Seo Il-Seon, Jung Young-Mi, Biotechnology Letters . 2000,第11期

机译：携带自身克隆的phbC基因的转化产碱杆菌的构建及聚-β-羟基丁酸生物合成的表征
3. Cloning of the Alcaligenes latus Polyhydroxyalkanoate Biosynthesis Genes and Use of These Genes for Enhanced Production of Poly(3-hydroxybutyrate) in Escherichia coli [J] . Jong-il Choi, Sang Yup Lee, Kyuboem Han Applied and Environmental Microbiology . 1998,第12期

机译：产碱菌聚羟基链烷酸酯生物合成基因的克隆及这些基因在大肠杆菌中增强聚（3-羟基丁酸酯）生产的用途
4. Production and Characterization of Polyhydroxyalkanoates (PHAs) from Inexpensive Substrates by Alcaligenes latus [C] . C. Thammawong, K. Thongkhong, K. Iamtassana, International Conference on Smart Materials - Smart/Intelligent Materials and Nanotechnology . 2008

机译：来自alcaligenes Latus的廉价基材的多羟基烷烃（PHA）的生产和表征
5. Characterization and comparison of acrylamidases isolated from two different acrylamide-utilizing bacteria, Ralstonia eutropha AUM-01 and Geobacillus thermoglucosidasius AUT-01. [D] . Cha, Min-Seok. 2009

机译：从两种不同的利用丙烯酰胺的细菌，富营养小球藻（Ralstonia eutropha）AUM-01和热葡糖芽孢杆菌（Geobacillus thermoglucosidasius）AUT-01分离出的丙烯酰胺酶的特性和比较。
6. Cloning of the Alcaligenes latus Polyhydroxyalkanoate Biosynthesis Genes and Use of These Genes for Enhanced Production of Poly(3-hydroxybutyrate) in Escherichia coli [O] . Jong-Il Choi, Sang Yup Lee, Kyuboem Han 1999

机译：产碱菌聚羟基链烷酸酯生物合成基因的克隆及这些基因在大肠杆菌中增强聚（3-羟基丁酸酯）生产的用途
7. Cloning of the Alcaligenes latus Polyhydroxyalkanoate Biosynthesis Genes and Use of These Genes for Enhanced Production of Poly(3-hydroxybutyrate) in Escherichia coli [O] . Jong-Il Choi, Sang Yup Lee, Kyuboem Han 1999

机译：克隆阿尔卡利格氏菌羟基烷酸酯生物合成基因，并使用这些基因在大肠杆菌中加强聚（3-羟丁酸酯）的生产

Comparison of phbC genes cloned from Ralstonia eutropha and Alcaligenes latus for utilization in metabolic engineeringof polyhydroxyalkanoate biosynthesis

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