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首页> 外文期刊>Current therapeutic research, clinical and experimental. >An Increase in Phosphorylation and Truncation of Crystallin With the Progression of Cataracts
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An Increase in Phosphorylation and Truncation of Crystallin With the Progression of Cataracts

机译：随着白内障的发生，结晶蛋白的磷酸化和截短反应增加

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Background: Cataracts are the leading cause of blindness worldwide; however, there is no evidence regarding the direct formation of cataracts. At present, there is no treatment method other than surgery to prevent the formation or progression of cataracts.Objective: Understanding the protein changes during various stages of cataracts might help realize the mechanism of the formation and progression of cataracts.Methods: Lens materials were collected from cataract surgery. Cataracts were classified according to lens opacity using the gradation of the Lens Opacities Classification System. Lens proteins were separated by 2-dimensional polyacrylamide gel electrophoresis. Protein spots were visualized by Coomassie blue staining, and expression patterns were analyzed. Protein spots of interest were excised from 2-dimensional polyacrylamide gel electrophoresis gels, digested in situ with trypsin, and analyzed by mass spectrometry and liquid chromatographic tandem mass spectrometry.Results: Crystallin was the major protein in the cataract lens, and aA, (3B1, aB, and PA4 were the dominant types. Crystallin aB and p"A4 increased with the formation of lens opacity. Moreover, phosphorylation and truncation of these proteins increased with the progression of cataracts. Conclusion: Crystallin aB and PA4 and phosphorylation and truncation of crystallin in the lens might contribute to the formation of cataracts. In contrast, acetylation was not dominant in the progression of cataracts and did not play major role in the formation of cataracts.
机译：背景：白内障是全球失明的主要原因;但是，没有证据表明白内障直接形成。目前，除外科手术外，没有其他方法可以预防白内障的形成或发展。目的：了解白内障各个阶段的蛋白质变化可能有助于了解白内障形成和发展的机制。方法：收集晶状体材料来自白内障手术。白内障根据晶状体的不透明度使用镜片不透明度分类系统的等级进行分类。通过二维聚丙烯酰胺凝胶电泳分离透镜蛋白。通过考马斯亮蓝染色使蛋白斑点可视化，并分析表达模式。从二维聚丙烯酰胺凝胶电泳凝胶上切下感兴趣的蛋白斑点，用胰蛋白酶原位消化，并通过质谱和液相色谱串联质谱进行分析。结果：晶状体蛋白是白内障晶状体中的主要蛋白，而aA（3B1 ，aB和PA4是主要类型。晶状体aB和p“ A4随着晶状体混浊的形成而增加。此外，这些蛋白的磷酸化和截短随着白内障的发展而增加。晶状体中的晶状体蛋白可能有助于白内障的形成，相反，乙酰化在白内障的进展中并不占主导地位，并且在白内障的形成中不发挥主要作用。

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