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首页> 外文期刊>ACS Macro Letters >Determination of Molecular Weights in Polyelectrolyte Mixtures Using Polymer Translocation through a Protein Nanopore
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Determination of Molecular Weights in Polyelectrolyte Mixtures Using Polymer Translocation through a Protein Nanopore

机译：使用聚合物通过蛋白质纳米孔的移位测定聚电解质混合物中的分子量

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摘要

We introduce a single molecular analysis technique for the evaluation of molecular weight distributions of polyelectrolyte solutions by measuring translocation times of sodium polystyrenesulfonate (NaPSS) chains in a mixture passing through an α-hemolysin protein nanopore. The ionic current through an α-hemolysin nanopore is partially blocked transiently when the pore is occupied by a polymer chain with an average residence time proportional to the molecular weight of the polymer chain. We have measured the translocation times for an equimolar mixture of four different molecular weight NaPSS standards and observed distinct translocation time distribution peaks, each of which corresponding to the different components in the mixture. Size exclusion chromatography analyses were performed on the equimolar and equiweight NaPSS mixtures of the same components and compared with the translocation time measurements. The experimental results demonstrate that measuring translocation times can be a competitive technique for estimating the broad molecular weight distributions of polyelectrolytes.
机译：我们通过测量通过α-溶血素蛋白纳米孔的混合物中聚苯乙烯磺酸钠（NaPSS）链的移位时间，介绍了一种用于评估聚电解质溶液分子量分布的单分子分析技术。当孔被聚合物链占据时，通过α-溶血素纳米孔的离子电流被部分瞬态阻断，其平均停留时间与聚合物链的分子量成正比。我们已经测量了四种不同分子量的NaPSS标准品的等摩尔混合物的转运时间，并观察到了明显的转运时间分布峰，每个峰均对应于混合物中的不同组分。对相同成分的等摩尔和等重NaPSS混合物进行尺寸排阻色谱分析，并与易位时间测量结果进行比较。实验结果表明，测量移位时间可能是一种竞争性技术，可用于估计聚电解质的宽分子量分布。

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