Introduction of the anti-apoptotic baculovirus p35 gene in passion fruit induces herbicide tolerance, reduced bacterial lesions, but does not inhibits passion fruit woodiness disease progress induced by cowpea aphid-borne mosaic virus (CABMV)

de Freitas DS; Coelho MCF; Souza MT; Marques A; Ribeiro BM

首页> 外文期刊>Biotechnology Letters >Introduction of the anti-apoptotic baculovirus p35 gene in passion fruit induces herbicide tolerance, reduced bacterial lesions, but does not inhibits passion fruit woodiness disease progress induced by cowpea aphid-borne mosaic virus (CABMV)

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Introduction of the anti-apoptotic baculovirus p35 gene in passion fruit induces herbicide tolerance, reduced bacterial lesions, but does not inhibits passion fruit woodiness disease progress induced by cowpea aphid-borne mosaic virus (CABMV)

机译：在百香果中引入抗凋亡杆状病毒p35基因可诱导除草剂耐受性，减少细菌损伤，但不抑制cow豆蚜虫花叶病毒（CABMV）诱导的百香果木质性疾病的进展

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The introduction of anti-apoptotic genes into plants leads to resistance to environmental stress and broad-spectrum disease resistance. The anti-apoptotic gene (p35) from a baculovirus was introduced into the genome of passion fruit plants by biobalistics. Eleven regenerated plants showed the presence of the p35 gene by PCR and/or dot blot hybridization. Transcriptional analysis of regenerated plants showed the presence of specific p35 transcripts in 9 of them. Regenerated plants containing the p35 gene were inoculated with the cowpea aphid-borne mosaic virus (CABMV), the bacterium Xanthomonas axonopodis pv passiflorae, and the herbicide, glufosinate, (Syngenta). None of the plants showed resistance to CABMV. Regenerated plants (p35+) showed less than half of local lesions showed by non-transgenic plants when inoculated with X. axonopodis and some p35+ plants showed increased tolerance to the glufosinate herbicide when compared to non-transgenic plants.

机译：将抗凋亡基因引入植物导致对环境胁迫的抗性和广谱抗病性。通过生物平衡技术将杆状病毒的抗凋亡基因（p35）引入百香果植物的基因组中。通过PCR和/或斑点印迹杂交，十一株再生植物显示p35基因的存在。再生植物的转录分析显示其中9个中存在特定的p35转录本。用containing豆蚜虫传播的花叶病毒（CABMV），细菌Xanthomonas axonopodis pv passiflorae和除草剂草铵膦（Syngenta）接种含有p35基因的再生植物。没有植物显示出对CABMV的抗性。与非转基因植物相比，再生植物（p35 +）接种轴突线虫时显示出非转基因植物不到一半的局部病害，一些p35 +植物对草铵膦除草剂的耐受性增强。

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《Biotechnology Letters》 |2007年第1期|共9页
作者
de Freitas DS; Coelho MCF; Souza MT; Marques A; Ribeiro BM;
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正文语种 eng
中图分类生物工程学（生物技术）;
关键词
apoptosis; baculovirus; herbicide tolerance; Passiflora; p35 gene; ANTIAPOPTOTIC GENES; ABIOTIC STRESS; EXPRESSION;

机译：细胞凋亡;杆状病毒;除草剂耐受性;西番莲;p35基因;抗凋亡基因;生物应激;表达;

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1. Introduction of the anti-apoptotic baculovirus p35 gene in passion fruit induces herbicide tolerance, reduced bacterial lesions, but does not inhibits passion fruit woodiness disease progress induced by cowpea aphid-borne mosaic virus (CABMV) [J] . de Freitas DS, Coelho MCF, Souza MT, Biotechnology Letters . 2007,第1期

机译：在百香果中引入抗凋亡杆状病毒p35基因可诱导除草剂耐受性，减少细菌损伤，但不抑制cow豆蚜虫花叶病毒（CABMV）诱导的百香果木质性疾病的进展
2. Yellow passion fruit genotypes reaction to woodiness disease (Cowpea aphid-borne mosaic virus) in field conditions [J] . O. S. Miranda, O.K. Yamanishi, J.R. Peixoto Acta Horticulturae . 2020,第1282期

机译：黄色激情果实基因型对木质疾病（豇豆蚜虫的病毒）在现场条件下的反应
3. Resistance to Cowpea aphid-borne mosaic virus in species and hybrids of Passiflora: advances for the control of the passion fruit woodiness disease in Brazil [J] . Santos Eileen Azevedo, Viana Alexandre Pio, de Oliveira Freitas Josie Cloviane, European Journal of Plant Pathology . 2015,第1期

机译：对西番莲种和杂种中Cow豆蚜虫传播的花叶病毒的抗性：控制巴西百香果木本病的研究进展
4. Transcriptome Sequencing Reveals a Complete Genome Sequence of Cowpea Aphid-Borne Mosaic Virus from Passion Fruit in Kenya [O] . F. Munguti, S. Maina, E. N. Nyaboga, 2019

机译：转录组测序揭示了肯尼亚百香果中pea豆蚜虫花叶病毒的完整基因组序列
5. Introduction of the anti-apoptotic baculovirus p35 gene in passion fruit induces herbicide tolerance, reduced bacterial lesions, but does not inhibits passion fruit woodiness disease progress induced by cowpea aphid-borne mosaic virus (CABMV [O] . Freitas, D.S., Coelho, M.C.F., Souza, M.T., 2007

机译：在百香果中引入抗凋亡杆状病毒p35基因可诱导除草剂耐受性，减少细菌损伤，但不抑制由cow豆蚜虫花叶病毒（CABMV）诱导的百香果木质性疾病的进展

Introduction of the anti-apoptotic baculovirus p35 gene in passion fruit induces herbicide tolerance, reduced bacterial lesions, but does not inhibits passion fruit woodiness disease progress induced by cowpea aphid-borne mosaic virus (CABMV)

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