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首页> 外文期刊>American Journal of Veterinary Research >Characterization of IgE-mediated cutaneous immediate and late-phase reactions in nonallergic horses.
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Characterization of IgE-mediated cutaneous immediate and late-phase reactions in nonallergic horses.

机译：非过敏性马中IgE介导的皮肤即时和晚期反应的特征。

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Objective - To characterize the response of skin of nonallergic horses following ID injection of polyclonal rabbit anti-canine IgE (anti-IgE) and rabbit IgG. Animals - 6 healthy horses. Procedures - Skin in the cervical area was injected ID with anti-IgE and IgG. Wheal measurements and skin biopsy specimens were obtained before and 20 minutes and 6, 24, and 48 hours after injection. Tissue sections were evaluated for inflammatory cells at 4 dermal depths. Immunohistochemical analysis for CD3, CD4, and CD8 was performed, and cell counts were evaluated. Results - Anti-IgE wheals were significantly larger than IgG wheals at 20 minutes and 6 and 24 hours after injection. There were significantly more degranulated mast cells after anti-IgE injection than after IgG injection. There were significantly more eosinophils at 6, 24, and 48 hours and neutrophils at 6 hours after anti-IgE injection, compared with cell numbers at those same times after IgG injection. There were significantly more eosinophils in the deeper dermis of anti-IgE samples, compared with results for IgG samples. No significant differences between treatments were detected for CD3 +, CD4 +, or CD8 + cells. Conclusions and Clinical Relevance - Injection of anti-IgE antibodies was associated with the development of gross and microscopic inflammation characterized by mast cell degranulation and accumulation of inflammatory cells, particularly eosinophils and neutrophils. This pattern appeared to be similar to that of horses with naturally developing allergic skin disease, although lymphocytes were not increased; thus, ID injection of anti-IgE in horses may be of use for evaluating allergic skin diseases of horses.
机译：目的-表征ID注射多克隆兔抗犬IgE（anti-IgE）和兔IgG后非过敏性马的皮肤反应。动物-6匹健康的马。程序-颈部区域皮肤内注射抗IgE和IgG。注射前，注射后20分钟，6分钟，24小时和48小时获得了鲸的测量结果和皮肤活检标本。评价组织切片在4个真皮深度的炎性细胞。进行了CD3，CD4和CD8的免疫组织化学分析，并评估了细胞计数。结果-注射后20分钟，6小时和24小时，抗IgE团明显大于IgG团。注射抗IgE后的肥大细胞明显比注射IgG后多。与注射IgG后相同时间的细胞数相比，在注射IgE后第6、24和48小时的嗜酸性粒细胞和第6小时的嗜中性粒细胞明显增多。与IgG样品的结果相比，抗IgE样品深层真皮中的嗜酸性粒细胞明显增多。对于CD3 +，CD4 +或CD8 +细胞，未发现治疗之间的显着差异。结论与临床相关性-注射抗IgE抗体与以肥大细胞脱粒和炎性细胞（特别是嗜酸性粒细胞和嗜中性粒细胞）蓄积为特征的肉眼和微观炎症的发展有关。尽管淋巴细胞没有增加，但这种模式似乎与自然发生过敏性皮肤病的马相似。因此，在马中注射抗IgE的ID可用于评估马的过敏性皮肤病。

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