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首页> 外文期刊>American Journal of Veterinary Research >Whole-body phenylalanine kinetics and skeletal muscle protein signaling in horses with pituitary pars intermedia dysfunction.
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Whole-body phenylalanine kinetics and skeletal muscle protein signaling in horses with pituitary pars intermedia dysfunction.

机译：患有垂体中间间隙功能障碍的马的全身苯丙氨酸动力学和骨骼肌蛋白信号传导。

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Objective - To compare whole-body phenylalanine kinetics and the abundance of factors in signaling pathways associated with skeletal muscle protein synthesis and protein breakdown between horses with pituitary pars intermedia dysfunction (PPID) and age-matched control horses without PPID. Animals - 12 aged horses (6 horses with PPID and 6 control horses; mean age, 25.0 and 25.7 years, respectively). Procedures - Plasma glucose, insulin, and amino acids concentrations were determined before and 90 minutes after feeding. Gluteal muscle biopsy samples were obtained from horses 90 minutes after feeding, and the abundance and activation of factors involved in signaling pathways of muscle protein synthesis and breakdown were determined. The next day, horses received a priming dose and 2 hours of a constant rate infusion of 13C sodium bicarbonate followed by a priming dose and 4 hours of a constant rate infusion of 1- 13C phenylalanine IV; whole-body protein synthesis was determined. Results - Plasma glucose and insulin concentrations were higher after feeding than they were before feeding for both groups of horses; however, no significant postprandial increase in plasma amino acids concentrations was detected for either group. Phenylalanine flux, oxidation, release from protein breakdown, and nonoxidative disposal were not significantly different between groups. No significant effect of PPID status was detected on the abundance or activation of positive or negative regulators of protein synthesis or positive regulators of protein breakdown. Conclusions and Clinical Relevance - Results of this study suggested that whole-body phenylalanine kinetics and the postprandial activation of signaling pathways that regulate protein synthesis and breakdown in muscles were not affected by PPID status alone in aged horses.
机译：目的-比较患有垂体中间性功能障碍（PPID）的马匹与年龄匹配的无PPID的马之间的全身苯丙氨酸动力学以及信号通路中与骨骼肌蛋白质合成和蛋白质分解相关的因素的丰富性。动物-12匹老年马（6匹具有PPID的马和6匹对照马;平均年龄分别为25.0和25.7岁）。程序-喂食前和喂食后90分钟测定血浆葡萄糖，胰岛素和氨基酸浓度。进食后90分钟从马获得臀肌活检样品，并确定参与肌肉蛋白质合成和分解的信号通路的因子的丰度和活化。第二天，马接受初剂量和2小时的13C碳酸氢钠恒定速率输注，然后初次剂量和4小时的1-13C苯丙氨酸IV恒定速率输注;确定了全身蛋白质合成。结果-两组马的摄食后血浆葡萄糖和胰岛素浓度均高于摄食前。但是，两组均未发现餐后血浆氨基酸浓度显着增加。两组之间的苯丙氨酸通量，氧化，蛋白质分解释放和非氧化处置均无显着差异。没有检测到PPID状态对蛋白合成的正或负调节剂或蛋白分解的正调节剂的丰度或活化没有显着影响。结论和临床意义-这项研究的结果表明，在成年马中，单独的PPID状态不会影响全身苯丙氨酸动力学和调节蛋白质合成和分解的信号通路的餐后激活。

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