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首页> 外文期刊>Biotechnology Letters >Characterization of beta-xylosidase from Thermoanaerobacterium thermosaccharolyticum and its application to the production of ginsenosides Rg(1) and Rh-1 from notoginsenosides R-1 and R-2
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Characterization of beta-xylosidase from Thermoanaerobacterium thermosaccharolyticum and its application to the production of ginsenosides Rg(1) and Rh-1 from notoginsenosides R-1 and R-2

机译：嗜热厌氧厌氧杆菌的β-木糖苷酶的表征及其在三七皂苷R-1和R-2中生产人参皂苷Rg（1）和Rh-1的应用

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摘要

beta-Xylosidase from Thermoanaerobacterium thermosaccharolyticum was purified by His-trap affinity chromatography giving a specific activity of 5.15 U mg(-1). From gel-filtration chromatography, the purified enzyme was a tetramer with a total molecular mass of 245 kDa. Maximal enzyme activity using o-nitrophenyl(NP)-beta-d-xylopyranoside was at pH 6.5 and 60 A degrees C, with a half-life of 50 h. The enzyme had highest activity for oNP-beta-d-xylopyranoside among aryl-glycosides, and was only active for notoginsenosides R-1 and R-2 amongst various ginsenosides. beta-Xylosidase completely converted 2 g notoginsenosides R-1 and R-2 l(-1) to 1.69 g ginsenoside Rg(1) l(-1) and 1.63 g ginsenoside Rh-1 l(-1) in 4 and 18 h, respectively, with molar conversion yields of 100 % and specific productivities of 0.21 and 0.05 g g-enzyme(-1) h(-1), respectively. To our knowledge, this is the first report on the enzymatic production of ginsenosides Rg(1) and Rh-1 from notoginsenosides R-1 and R-2
机译：通过His-trap亲和层析纯化热解嗜热厌氧杆菌的β-木糖苷酶，得到的比活性为5.15 U mg（-1）。根据凝胶过滤色谱，纯化的酶是四聚体，总分子量为245 kDa。使用邻硝基苯基（NP）-β-d-吡喃吡喃糖苷的最大酶活性为pH 6.5和60 A摄氏度，半衰期为50小时。该酶对芳基糖苷中的oNP-β-d-吡喃吡喃糖苷具有最高的活性，并且仅对各种人参皂苷中的三七皂苷R-1和R-2具有活性。 β-木糖苷酶在4和18小时内将2 g三七皂苷R-1和R-2 l（-1）完全转化为1.69 g人参皂苷Rg（1）l（-1）和1.63 g人参皂苷Rh-1 l（-1） ，摩尔转化率分别为100％和比生产率分别为0.21和0.05 g g-酶（-1）h（-1）。据我们所知，这是关于从三七皂苷R-1和R-2酶促生产人参皂苷Rg（1）和Rh-1的第一份报告

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