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首页> 外文期刊>Amino acids >Immunocytochemical study by two photon fluorescence microscopy of the distribution of GABAA receptor subunits in rat cerebellar granule cells in culture
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Immunocytochemical study by two photon fluorescence microscopy of the distribution of GABAA receptor subunits in rat cerebellar granule cells in culture

机译：通过两个光子荧光显微镜对培养的大鼠小脑颗粒细胞中GABAA受体亚单位分布的免疫细胞化学研究

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摘要

An immunocytochemical investigation of the expression of α_1, α_6, β_(2/3), γ_2 and subunits was performed on rat cerebellum granule cells in culture by the two photon microscopy technique. The first four subunits appear to be expressed abundantly in these cells, whereas the δ one seems to be expressed at a lower level. Another major difference in the distribution of these subunits is that whereas α_6, β_(2/3) and γ_2 appear only on plasma membranes 1 and are present mainly in the cell bodies cytoplasm. Still another difference was found in that the presence of γ_ on neurites is polarized, preferentially labelling neurites with the appearance of dendrites. The subunits α_6 and β_(2/3) appear to label all types of neurites, with β_(2/3) being by far the most heavily expressed subunit type. A final distinct characteristic is that α_6 and, even more, α_2 appear to accumulate in the cytoplasmic domains immediately below the cone of emergence of neurites. This suggests a conspicuous transport of such subunits from the site of synthesis in the cell body to the site of final expression in the neurites (dendrites and axon terminals).
机译：用两种光子显微镜技术对培养的大鼠小脑颗粒细胞进行了α_1，α_6，β_（2/3），γ_2及其亚基表达的免疫细胞化学研究。前四个亚基似乎在这些细胞中大量表达，而δ亚基似乎以较低水平表达。这些亚基分布的另一个主要区别是，α_6，β_（2/3）和γ_2仅出现在质膜1上，主要存在于细胞体的细胞质中。还发现另一个不同之处在于，神经突上存在γ_是极化的，优先标记带有树突的神经突。亚基α_6和β_（2/3）似乎标记了所有类型的神经突，其中β_（2/3）是迄今为止表达最多的亚基类型。最终的明显特征是α_6，甚至更多的α_2似乎在神经突出现锥的正下方的胞质域中积聚。这表明这些亚基从细胞体内的合成位点到神经突（树突和轴突末端）的最终表达位点明显转移。

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