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首页> 外文期刊>Amino acids >Hemolymph amino acid variations following behavioral and genetic changes in individual Drosophila larvae
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Hemolymph amino acid variations following behavioral and genetic changes in individual Drosophila larvae

机译：果蝇幼虫行为和遗传改变后血淋巴氨基酸变化

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摘要

This study investigated the effect of different sampling environments on hemolymph amino acid content of individual Drosophila melanogaster larvae. Hemolymph was collected from individual third instar larvae under cold-anesthetized, awake, and stress conditions. Qualitative and quantitative hemolymph amino acid analyses were performed via capillary electrophoresis with laser-induced fluorescence detection. The hemolymph amino acid concentrations, particularly arginine, glutamate, and taurine, changed significantly depending on the prior-to-sample-collection environments. Hemolymph amino acid analyses of six different Drosophila genotypes including two control genotypes and four mutant alleles were also carried out. Two mutant genotypes with over and under expression of a putative cystine-glutamate exchanger subunit were significantly different from each other with respect to their hemolymph glutamate, glycine, lysine, and taurine levels. Hemolymph amino acid analyses of stressed larvae of two control and two mutant genotypes indicated that behavior-related hemolymph chemical changes are also genotype dependent.
机译：本研究调查了不同采样环境对黑腹果蝇幼虫血淋巴氨基酸含量的影响。在冷麻醉，清醒和应激条件下从单个三龄幼虫中收集血淋巴。通过毛细管电泳和激光诱导的荧光检测对血淋巴氨基酸进行定性和定量分析。血淋巴氨基酸的浓度，尤其是精氨酸，谷氨酸和牛磺酸，根据样品采集前的环境而发生了显着变化。还进行了六种不同果蝇基因型的血淋巴氨基酸分析，包括两种对照基因型和四种突变等位基因。假定的胱氨酸-谷氨酸交换子亚基表达过高和不足的两种突变基因型在其血淋巴谷氨酸，甘氨酸，赖氨酸和牛磺酸水平方面彼此显着不同。对两个对照和两个突变基因型的应激幼虫的血淋巴氨基酸分析表明，行为相关的血淋巴化学变化也与基因型有关。

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