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首页> 外文期刊>American Journal of Orthodontics and Dentofacial Orthopedics >Microbial contamination of 'as received' and 'clinic exposed' orthodontic materials
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Microbial contamination of 'as received' and 'clinic exposed' orthodontic materials

机译：正畸材料“原样”和“暴露于临床”的微生物污染

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Introduction: Our objective was to determine whether components of fixed orthodontic appliances as received from the manufacturers and after exposure to the clinical environment are free from microbial contamination before clinical use. A pilot molecular microbiologic laboratory study was undertaken at a dental hospital in the United Kingdom. Methods: A range of orthodontic materials "as received" from the manufacturers and materials "exposed" to the clinical environment were studied for bacterial contamination. After growth on blood-rich media, cultured bacteria were identified by 16S rDNA polymerase chain reaction amplification and sequence phylogeny. Results: Bacteria were isolated from "as received" bands, archwires, and impression trays, but the level of contamination was low (0.5 × 10 1 to 1.825 × 102 CFU/mL-1). Various bacterial species were isolated from "clinic exposed" bands, archwires, impression trays, coil springs, and elastomeric modules, but the level of contamination was low (0.5 × 101 to 8.0 × 101 CFU/mL-1). The most commonly identified bacterial species was Staphylococcus epidermidis, followed by Kocuria, Moraxella, and Micrococcus species. Conclusions: New materials "as received" from the manufacturers and those exposed to the clinical environment are not free from bacterial contamination before use in patients, but this contamination is low considering the potential for aerosol and operator contamination and could be considered insignificant. Further studies would be required to determine the level of risk that this poses.
机译：简介：我们的目标是确定从制造商处获得的固定的正畸矫治器组件以及暴露于临床环境后的组件是否在临床使用前没有受到微生物污染。在英国的一家牙科医院进行了分子微生物实验室试验研究。方法：研究了制造商提供的一系列“正畸”材料和“暴露”于临床环境的材料是否受到细菌污染。在富血培养基上生长后，通过16S rDNA聚合酶链反应扩增和序列系统发育鉴定细菌。结果：细菌是从“原样”的条带，弓丝和印模托盘中分离出来的，但污染水平很低（0.5×10 1至1.825×102 CFU / mL-1）。从“暴露于临床的”条带，弓丝，印模托盘，螺旋弹簧和弹性体组件中分离出各种细菌，但污染水平较低（0.5×101至8.0×101 CFU / mL-1）。最常见的细菌种类是表皮葡萄球菌，其次是科库里亚，莫拉氏菌和微球菌。结论：从制造商处获得的新材料和暴露于临床环境的材料在用于患者之前并非没有细菌污染，但是考虑到气溶胶和操作员污染的可能性，这种污染很小，可以认为是微不足道的。需要进行进一步的研究以确定其带来的风险水平。

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