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HPLC 2012: New mysteries in column efficiency

机译:HPLC 2012:色谱柱效率的新谜团

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HPLC 2012, the 38th International Symposium on High Performance Liquid Phase Separations and Related Techniques, CA, June 16-21, 2012, highlighted latest advances in chromatography technology and applications. A lecture reported on the performance of experimental columns packed with 1.1-μm core/shell particles. Dr. Andy Alpert, President of PolyLC, Columbia, MD, and the founder of HILIC and related ERLIC presented a sunrise tutorial on orthogonal selectivity to reversed-phase liquid chromatography (RPLC) and improved detection sensitivity in LC-MS. Protocol for optimizing peak capacity for i.d. gradient elution separations of small molecules, starting with the restricted set of commercially available column dimensions, was also discussed. Two selective sorbents targeting phosphopeptides to improve MS detection and both were found to be effective in selective extraction of phosphopeptides.
机译:2012年6月16日至21日,在加利福尼亚州举行的第38届高性能液相分离和相关技术国际研讨会上,HPLC 2012着重介绍了色谱技术和应用的最新进展。一次讲座报告了填充有1.1μm核/壳颗粒的实验色谱柱的性能。 PolyLC总裁,哥伦比亚医学博士,HILIC和相关ERLIC的创建者Andy Alpert博士介绍了关于反相液相色谱(RPLC)的正交选择性和提高LC-MS检测灵敏度的日出教程。优化i.d.的峰值容量的协议还讨论了小分子的梯度洗脱分离方法,从有限的一组市售色谱柱尺寸开始。靶向磷酸肽的两种选择性吸附剂可改善MS检测,并且两种吸附剂均可有效地选择性提取磷酸肽。

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