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首页> 外文期刊>立命館大学理工学研究所紀要 >Metabolism of N-acetyl-D-amino acid by alcaligenes xylosoxydans subsp. xylosoxydans A-6
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Metabolism of N-acetyl-D-amino acid by alcaligenes xylosoxydans subsp. xylosoxydans A-6

机译：通过阿尔卡罗酮酸亚硫代甲苯甲基苯甲酸亚氨基甲酸氨基酸的N-乙酰基-D-氨基酸的代谢。 Xylosoxydans A-6

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摘要

We described the occurrence and properties of the enzymes engaged in the metabolism of D-amino acid formed from N-acetyl-D-amino acid by the N-acyl-D-amino acid amidohydrolase in Alcaligenes xylosoxydans subsp. xylosoxydans A-6 (Alcaligenes A-6). Oxidase, racemase, and transaminase activities toward D-amino acids were detected, but dehydrogenase activity wasn't. This result suggested that D-amino acid formed from N-acetyl-D-amino acid by N-acyl-D-amino acid amidohydrolase would be further degraded by these enzymes described above. Since Alcaligenes A-6 showed best growth on the medium containing N-acetyl-D-glutamate (D-Glu) among other N-acetyl-D-amino acids (D-aspartate, D-leucine) as a sole carbon and nitrogen source, the metabolism of N-acetyl-D-Glu, in particular, was investigated. D-Glu oxidase activity rapidly increased with the cell-growth re-increasing just after N-acetyl-D-Glu had been consumed in the medium, while high N-acyl-D-Glu amidohydrolase (D-AGase) activity was observed throughout the culture time. It seemed to be considered as the diauxie growth. D-Glu oxidase partially purified, which showed rather high substrate specificity and scarcely act on D-Asp, has been thought to be a membrane-bound enzyme.
机译：我们描述了通过N-酰基-D-氨基酸在阿尔卡罗代原亚氨基苯甲酰酶中由N-酰基-D-氨基酸形成为从N-乙酰基-D-氨基酸形成的D-氨基酸代谢的酶的发生和性质。 Xylosoxydans A-6（Alcaligenes A-6）。检测氧化酶，显影酶和转氨酶活性朝向D-氨基酸，但脱氢酶活性不是。该结果表明，通过N-酰基-D-氨基酸由N-乙酰基-D-氨基酸形成的D-氨基酸通过上述这些酶进一步降解。由于Alcaligenes A-6在含有N-乙酰-D-氨基酸（D-天冬氨酸，D-亮氨酸）中的含有N-乙酰基-D-谷氨酸（D-Glu）的培养基上的最佳生长，因此作为唯一的碳和氮源，研究了N-乙酰基-11Lu的代谢，特别是研究。 D-Glu氧化酶活性随着在培养基中消耗的N-乙酰基-D-glu之后，在N-乙酰基-DLU之后再增加而迅速增加，而始终观察到高N-酰基-D-Glu酰胺水（D-丙酶）活性文化时间。它似乎被视为静态的生长。部分纯化的D-glu氧化酶，其显示出相当高的底物特异性并几乎没有对D-ASP作用，被认为是膜结合的酶。

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