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首页> 外文期刊>立命館大学理工学研究所紀要 >Analysis of biosurfactant from bacillus sp. ODTY34
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Analysis of biosurfactant from bacillus sp. ODTY34

机译：芽孢杆菌生物活性剂分析。 Otty34.

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摘要

Waste oil from car engines degrading bacteria were isolated with LB medium containing 1% w/v waste oil. More than 20 bacteria were isolated as waste oil emulsifying bacteria. One bacterium, which was identified as Bacillus sp., strongly emulsified waste oil. The biosurfactant (BSF) activity of Bacillus sp. ODTY34 was 388 units/ml in LB medium after 22.5 hrs cultivation, and the BSF activity was enhanced by addition of glucose or glycerol. The BSF was purified by column and thin-layer chromatography. Molecular weight of the BSF was 1054.7 by mass spectrometry, and amino acids sequence of the BSF was L-Glu-L-Leu-D-Leu-L-Val-L-Asp-D-Leu-L-Leu, indicating that the BSF from strain ODTY34 was surfactin.
机译：来自汽车发动机降解细菌的废油与含有1％w / v废油的LB培养基分离。 将超过20个细菌作为废油乳化细菌分离。 一种细菌，被鉴定为芽孢杆菌。，强烈乳化的废油。 Bacillus sp的生物活性剂（BSF）活性。 Otty34在22.5小时培养后为LB培养基中的388个单位/ mL，通过添加葡萄糖或甘油增强了BSF活性。 通过柱和薄层色谱纯化BSF。 BSF的分子量为1054.7质谱法，BSF的氨基酸序列是L-Glu-L-Leu-d-Leu-L-Val-L-ASP-D-Leu-L-Leu，表明 来自菌株Odty34的BSF是Surfactin。

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