Induction of endophytic colonization in rice (Oryza sativa L.) tissue culture plants by Azorhizobium caulinodans

Senthilkumar M.; Madhaiyan M.; Sundaram S. P.; Sangeetha H.; Kannaiyan S.

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Induction of endophytic colonization in rice (Oryza sativa L.) tissue culture plants by Azorhizobium caulinodans

机译：满江红假单胞菌诱导水稻（Oryza sativa L.）组织培养植物内生菌落

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Endophytic colonization in rice was induced using rhizobia. Dehusked seeds of rice hybrid, CORH2, were used as explants for induction of calli. MS medium was modified with 2,4-D (2.5 mg lp#) and kinetin (0.2 mg lp#) for callus induction. Well-developed calli were inoculated with Azorhizobium caulinodans strains ORS 571 and AA-SK-5 by means of imbibition. All treated calli had significant increases in protein content, total nitrogen and nitrogenase activity. Imbibition of ORS 571 had significant biochemical effect on the developing calli than AA-SK-5. The crop response study from the regenerated plantlets showed a positive correlation in yield than uninoculated control. The endophytic colonization was observed in all parts of the plants analyzed. Further, colonization was also confirmed by microtome sectioning.

机译：利用根瘤菌诱导水稻内生定居。水稻杂种CORH2的去壳种子用作诱导愈伤组织的外植体。用2，4-D（2.5mg lp＃）和激动素（0.2mg lp＃）修饰MS培养基以诱导愈伤组织。用吸水法将成熟的愈伤组织接种了满江红假单胞菌caulinodans菌株ORS 571和AA-SK-5。所有处理过的愈伤组织的蛋白质含量，总氮和固氮酶活性均显着增加。与AA-SK-5相比，ORS 571的吸收对发育中的愈伤组织具有显着的生化作用。再生苗的农作物响应研究显示，产量比未接种对照正相关。在所分析植物的所有部分均观察到内生菌落。此外，还通过切片机切片确认了定植。

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《Biotechnology Letters》 |2008年第8期|共11页
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Senthilkumar M.; Madhaiyan M.; Sundaram S. P.; Sangeetha H.; Kannaiyan S.;
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正文语种 eng
中图分类生物工程学（生物技术）;生命科学总论;分子生物学;
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1. Production of bacterial blight resistant lines from somatic hybridization between Oryza sativa L. and Oryza meyeriana L. [J] . 严成其, 钱凯先, 薛刚平, 浙江大学学报：自然科学英文版 . 2004,第010期
2. Induction of endophytic colonization in rice (Oryza sativa L.) tissue culture plants by Azorhizobium caulinodans [J] . Senthilkumar M., Madhaiyan M., Sundaram S. P., Biotechnology Letters . 2008,第8期

机译：满江红假单胞菌诱导水稻（Oryza sativa L.）组织培养植物内生菌落
3. The xylem of rice (Oryza sativa) is colonized by Azorhizobium caulinodans. [J] . Gopalaswamy G, Kannaiyan S, OCallaghan KJ, The Royal Society Proceedings B: Biological Sciences . 2000,第1439期

机译：水稻的木质部被固氮假单胞菌定殖。
4. Dinitrogen fixation activity of Azorhizobium caulinodans in the rice (Oryza sativa L.) rhizosphere assessed by nitrogen balance and nitrogen-15 dilution methods [J] . C. Van Nieuwenhove, R. Merckx, Lieven Van Holm, Biology and Fertility of Soils . 2001,第1期

机译：用氮平衡和氮15稀释法评估水稻（Oryza sativa L.）根际中拟南芥属固氮菌的固氮活性
5. ROLE OF RHIZOBIAL ENDOPHYTES AS NITROGEN FIXER IN PROMOTING PLANT GROWTH AND PRODUCTIVITY OF INDIAN CULTIVATED UPLAND RICE (ORYZA SATIVA L.) PLANTS [C] . R. K. Singh, R. P. N. Mishra, H. K. Jaiswal International Nitrogen Fixation Congress . 2005

机译：Rhizobial Endophytes作为氮固定剂在促进植物生长和印度栽培旱稻生产率方面的作用（Oryza sativa L.）植物
6. Colonization of rice straw (Oryza sativa L.) by white-rot fungi: Effects on structure, chemical composition, cell wall components and nutrient digestibility in vitro. [D] . Karunanandaa, Kanthasamy. 1995

机译：白腐真菌对稻草（Oryza sativa L.）的定殖：对体外结构，化学成分，细胞壁成分和营养消化率的影响。
7. The xylem of rice (Oryza sativa) is colonized by Azorhizobium caulinodans. [O] . G Gopalaswamy, S Kannaiyan, K J OCallaghan, 2000

机译：水稻的木质部被固氮假单胞菌定殖。
8. The xylem of rice (Oryza sativa) is colonized by Azorhizobium caulinodans. [O] . Gopalaswamy, G, Kannaiyan, S, O'Callaghan, K J, 2000

机译：水稻的木质部被固氮假单胞菌定殖。

Induction of endophytic colonization in rice (Oryza sativa L.) tissue culture plants by Azorhizobium caulinodans

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