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首页> 外文期刊>Animal Science Journal >Conditions affecting growth and developmental competence of mammalian oocytes in vitro.
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Conditions affecting growth and developmental competence of mammalian oocytes in vitro.

机译：影响哺乳动物卵母细胞体外生长和发育能力的条件。

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摘要

Mammalian ovaries contain a large number of oocytes at different stages of growth. To utilize potential female gametes, it is important to develop culture systems that permit oocytes to achieve full growth and competence in order to undergo maturation, fertilization and development. The desired culture systems should meet at least the following three conditions: (i) oocytes remain healthy and functional so that they can execute intrinsic programs that direct their growth and development; (ii) granulosa cells that are adjacent to oocytes proliferate efficiently to prevent oocytes from becoming denuded; and (iii) granulosa cells maintain (and develop) appropriate associations with oocytes during the culture period. For this reason, several systems have been developed, and they can be classified into four categories based on the structure and components of the follicle/oocyte-granulosa cell complex and the location of the oocyte in the physical organization of the complex. The resultant diverse morphologies are due to multiple factors, including the method for initial isolation of follicles, the culture substrate, and hormones and other factors added into the medium. It is important to find an optimal combination of such factors involved in the process to facilitate future research efforts.Digital Object Identifier http://dx.doi.org/10.1111/j.1740-0929.2010.00870.x
机译：哺乳动物卵巢在不同的生长阶段包含大量卵母细胞。为了利用潜在的雌配子，重要的是要开发允许卵母细胞充分生长和发育的培养系统，以使其成熟，受精和发育。所需的培养系统应至少满足以下三个条件：（i）卵母细胞保持健康和功能性，以便它们可以执行指导其生长和发育的内在程序; （ii）与卵母细胞相邻的颗粒细胞有效增殖，以防止卵母细胞脱落。 （iii）颗粒细胞在培养期间与卵母细胞保持（并发展）适当的结合。因此，已经开发了几种系统，根据卵泡/卵母细胞-颗粒细胞复合物的结构和成分以及卵母细胞在复合物的物理组织中的位置，它们可以分为四类。最终产生的多种形态是由于多种因素引起的，包括最初分离卵泡的方法，培养底物以及激素和添加到培养基中的其他因素。重要的是找到过程中涉及的这些因素的最佳组合，以促进将来的研究工作。数字对象标识符http://dx.doi.org/10.1111/j.1740-0929.2010.00870.x

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