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首页> 外文期刊>Animal Science Journal >Oocytic expression of zona pellucida protein ZP4 in Japanese quail (Coturnix japonica).
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Oocytic expression of zona pellucida protein ZP4 in Japanese quail (Coturnix japonica).

机译：透明带蛋白ZP4在日本鹌鹑（ Coturnix japonica ）中的卵细胞表达。

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摘要

The avian perivitelline layer, an extracellular matrix homologous to the zona pellucida (ZP) of mammalian oocytes, is composed mainly by zona pellucida gene family glycoproteins. Our previous studies in Japanese quail have demonstrated that the matrix's components, ZP3 and ZPD, are synthesized in ovarian granulosa cells. Another component, ZP1, is synthesized in the liver. Recently, we demonstrated that another minor constituent, ZP2 is produced in the oocytes of the immature follicles. In the present study, we report the isolation of complementary DNA encoding quail ZP4 and its expression and origin in the female birds. By ribonuclease protection assay and in situ hybridization, we demonstrated that ZP4 transcripts were transcribed in the oocytes of small white follicles. The expression level of ZP4 decreased dramatically during follicular development, and the highest expression was observed in the small white follicles. Western blot analysis using the specific antibody against ZP4 indicated that the immunoreactive 58.2 kDa protein was present in the lysates of the small white follicles. These results demonstrate for the first time that the avian ZP4 is expressed in the oocyte, and that the expression pattern of the gene is similar to that of ZP2.
机译：禽卵周膜层是一种与哺乳动物卵母细胞透明带（ZP）同源的细胞外基质，主要由透明带基因家族糖蛋白组成。我们以前在日本鹌鹑中进行的研究表明，基质成分ZP3和ZPD是在卵巢颗粒细胞中合成的。肝脏中合成了另一种成分ZP1。最近，我们证明了在未成熟卵泡的卵母细胞中产生了另一种次要成分ZP2。在本研究中，我们报告了编码鹌鹑ZP4的互补DNA的分离及其在雌鸟中的表达和起源。通过核糖核酸酶保护试验和原位杂交，我们证明了ZP4转录物在小的白色卵泡的卵母细胞中转录。 ZP4的表达水平在卵泡发育过程中急剧下降，并且在小的白色卵泡中观察到最高的表达。使用针对ZP4的特异性抗体进行的蛋白质印迹分析表明，免疫反应性58.2 kDa蛋白存在于白色小卵泡的裂解物中。这些结果首次证明了禽ZP4在卵母细胞中表达，并且该基因的表达模式与ZP2相似。

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