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首页> 外文期刊>Biochemistry >Forward Photodynamics of the Noncanonical Red/Green NpR3784 Cyanobacteriochrome from Nostoc punctiforme
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Forward Photodynamics of the Noncanonical Red/Green NpR3784 Cyanobacteriochrome from Nostoc punctiforme

机译：来自Nostoc puncteriforme的非甘露花红/绿NPR3784胞间菌的前向光动力学

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摘要

Cyanobacteriochromes (CBCRs) make up a diverse family of cyanobacterial photoreceptors distantly related to the phytochrome photoreceptors of land plants. At least two lineages of CBCRs have reacquired red-absorbing dark states similar to the phytochrome P-r resting state but are coupled to green-absorbing light-adapted states rather than the canonical far-red-absorbing light-adapted state. One such lineage includes the canonical red/green (R/G) CBCRs that includes AnPixJg2 (UniProtKB Q8YXY7) and NpR6012g4 (UniProtKB B2IU14) that have been extensively characterized. Here we examine the forward Pr photodynamics of NpR3784 (UniProtKB B2J457), a representative member of the second R/G CBCR subfamily. Using broadband transient absorption pump-probe spectroscopy, we characterize both primary (100 fs to 10 ns) and secondary (10 ns to 1 ms) forward (P-r -> P-g) photodynamics and compare the results to temperature-jump cryokinetics measurements. Our studies show that primary isomerization dynamics occur on an similar to 10 p(s) timescale, yet remarkably, the red-shifted primary Lumi-R-f photoproduct found in all photoactive canonical R/G CBCRs examined to date is extremely short-lived in NpR3784. These results demonstrate that differences in reaction pathways reflect the evolutionary history of R/G CBCRs despite the convergent evolution of their photocycle end products.
机译：CyanobacterioChromes（CBCRS）构成了与土地植物的植物植物感光体远方的树状菌感受器多样化的蓝杆菌感光体。 CBCR的至少两个谱系具有类似于Phytochrome P-R静态状态的红色吸收的暗状态，而是耦合到绿色吸收光适应状态，而不是规范远红吸收光适应状态。一种这样的谱系包括具有广泛表征的ANPixJG2（UNIPROTKB Q8YXY7）和NPR6012G4（UNIPROTKB B2IU14）的规范红色/绿色（R / G）CBCR。在这里，我们检查NPR3784（UNIPROTKB B29457）的转发PR光动力学，第二个R / G CBCR亚家族的代表性成员。使用宽带瞬态吸收泵探针光谱，我们将主要（100 fs至10ns）和次级（10ns至1 ms）表征向前（p-r - > p-g）光动力学并将结果与​​温度跳过多语程测量进行比较。我们的研究表明，原发性异构化动态发生在类似于10 p（S）时间尺度上，但是，在迄今为止的所有光活性规范R / G CBCR中发现的红移原发性Lumi-RF光调节在NPR3784中非常短暂。 。这些结果表明，尽管它们的光细胞端产品的会聚演化，反应途径的差异反映了R / G CBCR的进化历史。

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  • 来源
    《Biochemistry》 |2019年第18期|共10页
  • 作者

    Kirpich Julia S.; Gottlieb Sean M.; Chang Che-Wei; Kim Peter W.; Martin Shelley S.; Lagarias J. Clark; Larsen Delmar S.;

  • 作者单位

    Univ Calif Davis Dept Chem One Shields Ave Davis CA 95616 USA;

    Univ Calif Davis Dept Chem One Shields Ave Davis CA 95616 USA;

    Univ Calif Davis Dept Chem One Shields Ave Davis CA 95616 USA;

    Univ Calif Davis Dept Chem One Shields Ave Davis CA 95616 USA;

    Univ Calif Davis Dept Mol &

    Cell Biol One Shields Ave Davis CA 95616 USA;

    Univ Calif Davis Dept Mol &

    Cell Biol One Shields Ave Davis CA 95616 USA;

    Univ Calif Davis Dept Chem One Shields Ave Davis CA 95616 USA;

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  • 正文语种 eng
  • 中图分类 生物化学;
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