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首页> 外文期刊>Biochemistry >Reverse Photodynamics of the Noncanonical Red/Green NpR3784 Cyanobacteriochrome from Nostoc punctiforme
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Reverse Photodynamics of the Noncanonical Red/Green NpR3784 Cyanobacteriochrome from Nostoc punctiforme

机译：来自Nostoc Puncteriforme的非甘露吞形红色/绿色NPR3784 Cyanobacteriochrome的反向光学动力学

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摘要

In the companion paper (10.1021/acs.biochem.8b01274), we examined the forward P-r photodynamics of NpR3784 (UniProtKB B2J457), a representative member of a noncanonical red/green (R/G) cyanobacteriochrome (CBCR) subfamily. Here the reverse P-g -> P-r photodynamics of NpR3784 was studied by broadband transient absorption pump-probe spectroscopy. Primary (100 fs to 10 ns) and secondary (10 ns to 1 ms) photodynamics were characterized over nine decades of time, which also were complemented with temperature-jump cryokinetics measurements. In contrast with canonical R/G CBCRs, the NpR3784 reverse photoconversion yielded two spectrally distinct primary photoproducts, Lumi-G(O) and Lumi-G(r) which decay on different time scales. The two primary photoproducts of NpR3784 equilibrate on the 40 ns time scale and subsequently propagate as a single intermediate population into P-r Such heterogeneity could arise from differences in the direction of D-ring rotation, in chromophore protonation or hydrogen bonding, or in the mobility of protein residues or of solvent water nearby the chromophore or some combination therein. We conclude that the atypical photodynamics of NpR3784 reflects chromophore-protein interactions that differ from those present in the canonical R/G CBCR family.
机译：在伴随论文（10.1021 / ACS.Biochem.8b01274）中，我们检查了NPR3784（Uniprotkb B29457）的前向P-R光动力学，非甘露吞噬/绿色（R / G）亚家族（CBCR）亚家族的代表性成员。这里，通过宽带瞬态吸收泵探针光谱研究了NPR3784的反向P-G - > P-R光动力学。初级（100fs至10ns）和次级（10ns至1ms）光动力学的特征在于九十年的时间，这也与温度跳过多发性测量相提并论。与规范R / G CBCR相比，NPR3784反向光电转化产生两种光谱不同的初级光调节，Lumi-G（O）和Lumi-G（R），其衰减在不同的时间尺度上。 NPR3784的两个初级光调节在40ns时间尺度上平衡并随后将作为单个中间人种群传播为Pr这样的异质性，可能出现在D形环旋转方向上的差异，在发色团质量或氢键，或在迁移率在发色团附近的蛋白质残留物或附近的溶剂水或其中一些组合。我们得出结论，NPR3784的非典型光动力学反映了与规范R / G CBCR家族中存在的色团蛋白质相互作用。

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  • 来源
    《Biochemistry》 |2019年第18期|共11页
  • 作者

    Kirpich Julia S.; Gottlieb Sean M.; Chang Che-Wei; Kim Peter W.; Martin Shelley S.; Lagarias J. Clark; Larsen Delmar S.;

  • 作者单位

    Univ Calif Davis Dept Chem One Shields Ave Davis CA 95616 USA;

    Univ Calif Davis Dept Chem One Shields Ave Davis CA 95616 USA;

    Univ Calif Davis Dept Chem One Shields Ave Davis CA 95616 USA;

    Univ Calif Davis Dept Chem One Shields Ave Davis CA 95616 USA;

    Univ Calif Davis Dept Mol &

    Cell Biol One Shields Ave Davis CA 95616 USA;

    Univ Calif Davis Dept Mol &

    Cell Biol One Shields Ave Davis CA 95616 USA;

    Univ Calif Davis Dept Chem One Shields Ave Davis CA 95616 USA;

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  • 正文语种 eng
  • 中图分类 生物化学;
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