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首页> 外文期刊>Biomaterials >Preservation of micro-architecture and angiogenic potential in a pulmonary acellular matrix obtained using intermittent intra-tracheal flow of detergent enzymatic treatment
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Preservation of micro-architecture and angiogenic potential in a pulmonary acellular matrix obtained using intermittent intra-tracheal flow of detergent enzymatic treatment

机译：使用间歇性酶促处理中的间歇性气管内流动获得的肺部造粒基质中的微结构和血管生成潜力

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Tissue engineering of autologous lung tissue aims to become a therapeutic alternative to transplantation. Efforts published so far in creating scaffolds have used harsh decellularization techniques that damage the extracellular matrix (ECM), deplete its components and take up to 5 weeks to perform. The aim of this study was to create a lung natural acellular scaffold using a method that will reduce the time of production and better preserve scaffold architecture and ECM components. Decellularization of rat lungs via the intratracheal route removed most of the nuclear material when compared to the other entry points. An intermittent inflation approach that mimics lung respiration yielded an acellular scaffold in a shorter time with an improved preservation of pulmonary micro-architecture. Electron microscopy demonstrated the maintenance of an intact alveolar network, with no evidence of collapse or tearing. Pulsatile dye injection via the vasculature indicated an intact capillary network in the scaffold. Morphometry analysis demonstrated a significant increase in alveolar fractional volume, with alveolar size analysis confirming that alveolar dimensions were maintained. Biomechanical testing of the scaffolds indicated an increase in resistance and elastance when compared to fresh lungs. Staining and quantification for ECM components showed a presence of collagen, elastin, GAG and laminin. The intratracheal intermittent decellularization methodology could be translated to sheep lungs, demonstrating a preservation of ECM components, alveolar and vascular architecture. Decellularization treatment and methodology preserves lung architecture and ECM whilst reducing the production time to 3 h. Cell seeding and in vivo experiments are necessary to proceed towards clinical translation.
机译：自体肺组织组织工程旨在成为移植的治疗替代品。到目前为止发布的努力在创造脚手架上使用了危害细胞外基质（ECM）的苛刻脱细胞化技术，耗尽其组分并占5周才能进行。本研究的目的是使用将减少生产时间和更好的保存脚手架架构和ECM组件的方法来创造肺天然牙科脚轮。与其他入选点相比，通过肿瘤内术通过肿瘤术的大鼠肺的脱细胞化除去了大部分核材料。模拟肺呼吸的间歇性膨胀方法在较短的时间内产生了无细胞支架，并改善了肺部微架构的保存。电子显微镜证明了维护完整的肺泡网络，没有崩溃或撕裂的证据。脉动染料通过脉管系统注射注射脚手架中的完整毛细管网络。形态学分析表明肺泡分数大幅增加，具有肺泡尺寸分析，证实保持肺泡尺寸。与新鲜肺相比，支架的生物力学测试表明耐药性和弹性增加。 ECM组分的染色和定量显示出胶原蛋白，弹性蛋白，GAG和层粘连蛋白的存在。肿瘤内间歇性脱细胞化方法可以转化为绵羊肺，证明了ECM组分，肺泡和血管结构的保存。脱细胞化处理和方法保留肺部建筑和ECM，同时将生产时间降至3小时。细胞播种和体内实验是进行临床翻译所必需的。

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