...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biophysical Journal >Comparison between whole distribution- and average-based approaches to the determination of fluorescence resonance energy transfer efficiency in ensembles of proteins in living cells.
【24h】

Comparison between whole distribution- and average-based approaches to the determination of fluorescence resonance energy transfer efficiency in ensembles of proteins in living cells.

机译：基于蛋白质蛋白酶集合的荧光共振能量转移效率的整体分布和平均方法的比较。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Current methods for analysis of data from studies of protein-protein interactions using fluorescence resonance energy transfer (FRET) emerged from several decades of research using wide-field microscopes and spectrofluorometers to measure fluorescence from individual cells or cell populations. Inherent to most measurements is an averaging of the distributions of FRET efficiencies over large populations of protein complexes, which washes out information regarding the stoichiometry and structure of protein complexes. Although the introduction of laser-scanning microscopes in principle could facilitate quantification of the distributions of FRET efficiencies in live cells, only comparatively recently did this potential fully materialize, through development of spectral- or lifetime-based approaches. To exploit this new opportunity in molecular imaging, it is necessary to further develop theoretical models and methods of data analysis. Using Monte Carlo simulations, we investigated FRET in homogenous and inhomogeneous spatial distributions of molecules. Our results indicate that an analysis based on distributions of FRET efficiencies presents significant advantages over the average-based approach, which include allowing for proper identification of biologically relevant FRET. This study provides insights into the effect of molecular crowding on FRET, and it offers a basis for information extraction from distributions of FRET efficiencies using simulations-based data fitting.
机译：通过使用宽野显微镜和光谱荧光计测量单个细胞或细胞种群的荧光共振能量转移（FRET）从研究蛋白质 - 蛋白质相互作用研究的数据分析了来自蛋白质 - 蛋白质相互作用的数据的分析方法。大多数测量的固有是在大型蛋白质复合物的大群体上的荧光效率分布的平均，这是关于化学计量和蛋白质复合物结构的信息。尽管原则上的激光扫描显微镜引入可以促进活细胞中荧光效率的分布，但最近通过开发基于频谱或终身的方法来实现这一潜在的潜在完全实现。为了利用分子成像的新机会，有必要进一步开发数据分析的理论模型和方法。使用Monte Carlo模拟，我们调查了分子均匀和不均匀的空间分布中的褶皱。我们的结果表明，基于FRET效率分布的分析具有与基于平均水平的方法的显着优势，包括允许正确识别生物相关的FRET。本研究提供了洞察分子挤在FRET上的效果，并且它为使用基于模拟的数据配件来提取FRET效率分布的基础。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号