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首页> 外文期刊>Bioresource Technology: Biomass, Bioenergy, Biowastes, Conversion Technologies, Biotransformations, Production Technologies >Optimization of an artificial cellulase cocktail for high-solids enzymatic hydrolysis of cellulosic materials with different pretreatment methods
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Optimization of an artificial cellulase cocktail for high-solids enzymatic hydrolysis of cellulosic materials with different pretreatment methods

机译：用不同预处理方法优化纤维素材料高固体酶水解的人工纤维素酶鸡尾酒

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摘要

Optimization of the composition of cellulase mixtures is an effective strategy to improve their hydrolytic efficiency and reduce protein demand during enzymatic degradation of lignocelluloses. In this study, the mixture design method was used to optimize the ratios of endoglucanase II (EG II), cellobiohydrolase I (CBH I) and beta-glucosidase I (BG I) from Penicillium oxalicum in an artificial cellulase mixture for the hydrolysis of six different cellulosic materials. The optimal composition of enzyme mixture was distinctly different among not only cellulosic materials with different pretreatment methods but hydrolyses at different solids concentrations. CBH I was most critical for the hydrolysis of two acid-pretreated materials, probably due to its strong adsorption on lignin. A higher proportion of EG II was needed for the hydrolysis of ammonium sulfite pretreated wheat straw. The requirements of specific cellulase components were more pronounced at high solids concentrations, highlighting the importance of considering solids loading when optimizing cellulase cocktails.
机译：纤维素酶混合物组合物的优化是提高其水解效率的有效策略，并降低木质纤维素酶促降解期间的蛋白质需求。在该研究中，混合物设计方法用于优化在人工纤维素酶混合物中的青霉属植物混合物中的内切葡聚糖酶II（例如II），纤维二氢酶I（CBH I）和β-葡糖苷酶I（BG I）的比例。不同的纤维素材料。酶混合物的最佳组成在不仅具有不同预处理方法的纤维素材料中明显不同，但在不同的固体浓度下水解。 CBH I最关键于水解两种酸预处理材料，可能是由于其对木质素的强烈吸附。亚硫酸铵预处理麦秸的水解需要更高比例的EG II。特定纤维素酶组分的要求在高固体浓度下更明显，突出了在优化纤维素酶鸡尾酒时考虑固体载荷的重要性。

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