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首页> 外文期刊>Bioresource Technology: Biomass, Bioenergy, Biowastes, Conversion Technologies, Biotransformations, Production Technologies >Enhanced reducing sugar production by saccharification of lignocellulosic biomass, Pennisetum species through cellulase from a newly isolated Aspergillus fumigatus
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Enhanced reducing sugar production by saccharification of lignocellulosic biomass, Pennisetum species through cellulase from a newly isolated Aspergillus fumigatus

机译：通过从新分离的曲霉菌的纤维素酶，通过纤维素酶，通过纤维素酶，通过纤维素酶进行糖化，通过纤维素酶进行增强降低糖生产

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摘要

A cellulose degrading fungus Aspergillus fumigatus (CWSF-7) isolated from decomposed lignocellulosic waste containing soil was found to produce high titer of cellulases. The optimum activity of CMCase and FPase were 1.9 U/mL and 0.9 U/mL respectively while the highest protein concentration was found to be 1.2 mg/mL. Saccharification of two Pennisetum grass varieties [dennanath (DG) and hybrid napier grass (HNG)] were optimized using partially purified CMCase and FPase in equal concentration, i.e. a ratios of 1:1 and further with addition of commercial xylanase using response surface methodology (RSM). The production of total reducing sugar (TRS) using isolated cellulase were 396.6 and 355.8 (mg/g), whereas further addition of xylanase had higher TRS titers of 478.7 and 483.3 (mg/g) for DG and HNG respectively as evident from HPLC analysis. Further, characterization of the enzyme saccharified DG and HNG by SEM and ATR-FTIR revealed efficient hydrolysis of cellulose and partially hydrolysis of hemicellulose.
机译：发现从含有含有土壤的分解的木质纤维素废物中分离的纤维素降解真菌（CWSF-7）产生高纤维素酶。 CMCase和FPase的最佳活性分别为1.9U / mL和0.9U / mL，而最高蛋白质浓度被发现为1.2mg / ml。使用部分纯化的CMCase和FPase的两种PenniSetum草种[Dennanath（DG）和杂交纳米尔草（HNG）]以等浓度的相等浓度的比例进行优化，即1：1的比例，并使用响应表面方法添加商业木聚糖酶（ RSM）。使用分离的纤维素酶生产总还原糖（TRS）为396.6和355.8（Mg / g），而另外加入木聚糖酶的TRS滴度为478.7和483.3（Mg / g）的TRS滴度，分别从HPLC分析中明显看出DG和HNG 。此外，SEM和ATR-FTIR的酶糖化DG和HNG的表征揭示了纤维素的有效水解和半纤维素的部分水解。

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