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首页> 外文期刊>Biochemical and Biophysical Research Communications >A vertebrate model for the study of lipid binding/transfer protein function: Conservation of OSBP-related proteins between zebrafish and human
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A vertebrate model for the study of lipid binding/transfer protein function: Conservation of OSBP-related proteins between zebrafish and human

机译：脂质结合/转移蛋白功能研究的脊椎动物模型：斑马鱼和人类之间的OSBP相关蛋白质的保护

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摘要

Oxysterol-binding protein (OSBP) and OSBP-related (ORP) or OSBP-like (OSBPL) proteins constitute a family of lipid-binding/transfer proteins (LTPs) present in eukaryotes from yeast to man. The mechanisms of ORP function have remained incompletely understood. However, several ORPs are present at membrane contact sites and act as either lipid transporters or sensors that control lipid metabolism, cell signaling, and vesicle transport. Zebrafish, Danio rerio, has gained increasing popularity as a model organism in developmental biology, human disease, toxicology, and drug discovery. However, LTPs in the fish are thus far unexplored. In this article we report a series of bioinformatic analyses showing that the OSBPL gene family is highly conserved between the fish and human. The OSBPL subfamily structure is markedly similar between the two organisms, and all 12 human genes have orthologs, designated osbpl and located on 11 chromosomes in D. rerio. Interestingly, osbpl2 and osbpl3 are present as two closely related homologs (a and b), due to gene duplication events in the teleost lineage. Moreover, the domain structures of the distinct ORP proteins are almost identical between zebrafish and man, and molecular modeling in the present study suggests that ORD liganding by phosphatidylinositol-4-phosphate (PI4P) is a feature conserved between yeast Osh3p, human ORP3, and zebrafish Osbpl3. The present analysis identifies D. rerio as an attractive model to study the functions of ORPs in vertebrate development and metabolism.
机译：氧食醇结合蛋白（OSBP）和OSBP相关（OSP）或OSBP样（OSBPL）蛋白质构成了从酵母到人的真核生物中存在的脂质结合/转移蛋白（LTPS）。 ORP功能的机制保持不完全理解。然而，膜接触位点存在几个ORPS，并充当控制脂质代谢，细胞信号和囊泡运输的脂质转运蛋白或传感器。 Zebrafish，Danio Rerio在发育生物学，人类疾病，毒理学和药物发现中增加了普及。然而，迄今为止，鱼类中的LTP是遥远的。在本文中，我们报告了一系列生物信息分析，表明OSBPL基因家族在鱼类和人之间高度保守。两种生物之间的OSBPL亚家族结构明显相似，并且所有12个人基因都有直肠，指定OSBPL，位于12个染色体上的D.Rerio。有趣的是，OSBPL2和OSBPL3由于紧邻谱系中的基因重复事件而作为两个密切相关的同源物（A和B）。此外，不同ORP蛋白的畴结构几乎与斑马鱼和人类之间的相同，本研究中的分子建模表明，通过磷脂酰肌醇-4-磷酸酯（PI4P）的ORD配体是酵母OSH3P，人ORP3和斑马鱼OSBPL3。本分析将D. Rerio作为研究ORPS在脊椎动物发育和新陈代谢中的功能的有吸引力的模型。

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