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首页> 外文期刊>Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices >Highly sensitive olfactory biosensors for the detection of volatile organic compounds by surface plasmon resonance imaging
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Highly sensitive olfactory biosensors for the detection of volatile organic compounds by surface plasmon resonance imaging

机译：高敏感的嗅觉生物传感器，用于通过表面等离子体共振成像检测挥发性有机化合物

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摘要

Nowadays, monitoring of volatile organic compounds (VOCs) is very important in various domains. In this work, we aimed to develop sensitive olfactory biosensors using odorant binding proteins (OBPs) as sensing materials. Three rat OBP3 derivatives with customized binding properties were designed and immobilized on the same chip for the detection of VOCs in solution by surface plasmon resonance imaging (SPRi). We demonstrated that the proteins kept their binding properties after the immobilization under optimized conditions. The obtained olfactory biosensors exhibited very low limits of detection in both concentration (200 pM of beta-ionone) and in molecular weight of VOCs (100 g/mol for hexanal). Such a performance obtained with SPRi in solution is especially remarkable. We hypothesized that the binding of VOCs to the active sites of OBPs induced a local conformational change in the proteins. This change would give rise to a variation of refractive index, to which SPRi is extremely sensitive. In addition, the olfactory biosensors showed a high selectivity especially at relatively low VOC concentrations. With optimized regeneration procedures, they also showed very good repeatability not only from measurement to measurement but also from chip to chip with a lifespan up to almost two months. These olfactory biosensors are particularly interesting for trace detection of VOCs in solution.
机译：如今，监测挥发性有机化合物（VOC）在各个结构域中非常重要。在这项工作中，我们旨在使用气味结合蛋白（OBP）作为传感材料开发敏感的嗅觉生物传感器。三种RAT OBP3衍生物具有定制结合性能的衍生物被设计和固定在同一芯片上，用于通过表面等离子体共振成像（SPRI）检测溶液中的VOC。我们证明，在优化条件下固定后，蛋白质保持其结合性质。所获得的嗅觉生物传感器在浓度（β-离子网）和VOC的分子量（己醛100g / mol）中表现出非常低的检测限。用SPRI在溶液中获得的这种性能尤其显着。我们假设VOC与OBPS活性位点的结合诱导蛋白质中的局部构象变化。这种变化会产生折射率的变化，SPRI非常敏感。此外，嗅觉生物传感器尤其显示出高的选择性，特别是在相对低的VOC浓度下。通过优化的再生程序，它们还表现出非常好的重复性，而不仅可以从测量到测量，而且从芯片到芯片，寿命高达近两个月。这些嗅觉生物传感器对溶液中的VOCs进行痕量途径特别有趣。

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