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首页> 外文期刊>British Journal of Dermatology >Photodynamic inactivation of bacteria to decolonize meticillin‐resistant Staphylococcus aureus Staphylococcus aureus from human skin
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Photodynamic inactivation of bacteria to decolonize meticillin‐resistant Staphylococcus aureus Staphylococcus aureus from human skin

机译：细菌的光动力灭活，从人体皮肤脱卵抗核苷酸金黄色葡萄球菌金黄色葡萄球菌

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Summary Background To prevent infections that arise from the skin surface it is necessary to decolonize human skin prior to any proposed treatment or surgical intervention. Photodynamic inactivation of bacteria ( PIB ) uses cationic photosensitizers that attach to the surface of bacteria, generate reactive oxygen species on light irradiation and thereby kill bacteria via oxidative mechanisms. Objectives To evaluate the potential and the safety of PIB for decolonization of bacteria from skin. Methods PIB with the new photosensitizer SAPYR [2‐((4‐pyridinyl)methyl)‐1H‐phenalen‐1‐one chloride] was initially tested against different bacterial species in vitro . Then, ex vivo porcine skin samples were used as a model for decolonization of different bacteria species. The numbers of viable bacteria were quantified and the mitochondrial activity of skin cells was histologically analysed (using nitroblue tetrazolium chloride, NBTC ). The same procedure was performed for human skin and meticillin‐resistant Staphylococcus aureus ( MRSA ). Results The in vitro studies showed a 5 log 10 reduction of all tested bacterial species. On ex vivo porcine skin samples, PIB reduced the viability of all tested bacterial species by at least 3 log 10 steps. On human skin samples ex vivo , PIB reduced the number of viable MRSA by maximal 4·4 log 10 steps (1000 μmol L ?1 SAPYR , incubation time 10 min, 60 J cm ?2 ). NBTC staining showed normal mitochondrial activity in skin cells after all PIB modalities. Conclusions The results of this study show that PIB can effectively and safely kill bacteria like MRSA on the skin surface and might have the potential of skin decolonization in vivo .
机译：摘要背景，以防止从皮肤表面产生的感染有必要在任何提出的治疗或手术干预之前脱卵。细菌的光动力灭活（PIB）使用附着于细菌表面的阳离子光敏剂，在光照射上产生反应性氧物质，从而通过氧化机制杀死细菌。目的评价PIB对皮肤细菌的脱殖的潜力和安全性。方法使用新的光敏剂SaPyr [2 - （（（4-吡啶基）甲基）-1H-吩丙基-1-一氯化物]对不同的细菌物种进行PIB。然后，将前体内猪皮肤样品用作不同细菌种类的非殖民化的模型。量化了活细菌的数量，并且皮肤细胞的线粒体活性在组织学分析（使用Nitroblue四唑氯化物，NBTC）。对人体皮肤和抗性耐金黄色葡萄球菌（MRSA）进行相同的程序。结果体外研究表明，所有测试的细菌种类的50％降低。在离体猪皮肤样品上，PIB通过至少3个LOG 10步骤降低了所有测试细菌种类的活力。在人体皮肤样品中，PIB通过最大4·4 log 10步骤减少了可行MRSA的数量（1000μmol，1 sapyr，孵育时间10分钟，60J厘米Δ2）。在所有PIB型号之后，NBTC染色显示皮肤细胞中的正常线粒体活性。结论本研究的结果表明，PIB可以有效和安全地杀死皮肤表面上的MRSA等细菌，并且可能具有体内皮肤脱髓鞘的潜力。

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  • 来源
    《British Journal of Dermatology》 |2018年第6期|共10页
  • 作者

    Schreiner M.; B?umler W.; Eckl D.B.; Sp?th A.; K?nig B.; Eichner A.;

  • 作者单位

    Department of DermatologyUniversity Hospital RegensburgFranz‐Josef‐Strauss‐Allee 11 93053;

    Department of DermatologyUniversity Hospital RegensburgFranz‐Josef‐Strauss‐Allee 11 93053;

    Institute of MicrobiologyUniversity of RegensburgRegensburg Germany;

    TriOptoTec GmbHAm Biopark 13 Regensburg Germany;

    Department of Organic ChemistryUniversity of RegensburgRegensburg Germany;

    Department of DermatologyUniversity Hospital RegensburgFranz‐Josef‐Strauss‐Allee 11 93053;

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  • 正文语种 eng
  • 中图分类 皮肤病学与性病学;
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