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Biomimetic Mineralization of Protein Nanogels for Enzyme Protection

机译：酶保护蛋白纳米凝胶的仿生矿化

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摘要

Protein nanogels have found a wide variety of applications, ranging from biocatalysis to drug/protein delivery. However, in practical applications, proteins in nanogels may suffer from enzymic hydrolysis and denaturation. Inspired by the structure and functionalities of the fowl eggshells, biomimetic mineralization of protein nanogels was studied in this research. Protein nanogels with embedded porcine pancreas lipase (PPL) in the cross-linked nanostructures were synthesized through the thiol–disulfide reaction between thiol-functionalized PPL and poly(N-isopropylacrylamide) with pendant pyridyl disulfide groups. The nanogels were further reacted with reduced bovine serum albumin (BSA) and BSA molecules were coated on the nanogels. Mineralization of BSA leads to the synthesis of biomineralized shells on the nanogels. With the growth of CaCO3 on the shells, the nanogels aggregate into suprastructures. Thermogravimetric analysis, XRD, dynamic light scattering, and TEM were employed to study the mechanism of the biomineralization process and analyze the structures of the mineralized nanogels. The biomineralized shells can effectively protect the PPL molecules from hydrolysis by trypsin; meanwhile, the nanosized channels on the mineralized shells allow the transport of small-molecule substrates across the shells. Bioactivity measurements indicate that PPL in the nanogels maintains more than 80% bioactivity after biomineralization.
机译：蛋白质纳米凝胶已发现各种各样的应用，从生物分析到药物/蛋白质递送。然而，在实际应用中，纳米凝胶中的蛋白质可能患有酶水解和变性。灵感来自鸡蛋壳的结构和功能，在本研究中研究了蛋白质纳米凝胶的仿生矿化。通过脊髓官能化的PPL和聚（N-异丙基丙烯酰胺）与侧链吡啶基二硫基团的硫醇二硫化物反应合成具有嵌入的猪胰腺脂肪酶（PPL）的蛋白质纳米粒子。将纳米凝胶与将牛血清白蛋白（BSA）的还原物进一步反应，并在纳米凝胶上涂覆BSA分子。 BSA的矿化导致在纳米凝胶上的生物抗体壳的合成。随着CaCO3在壳体上的生长，纳米凝胶聚集成超法。采用热重分析，XRD，动态光散射和温度研究生物矿化过程的机制，分析矿化纳米凝胶的结构。生物丙原化壳可以通过胰蛋白酶有效保护PPL分子免受胰蛋白酶的水解;同时，矿化壳上的纳米通道允许在壳体上运输小分子底物。生物活性测量表明纳米凝胶中的PPL在生物矿化后维持了80％以上的生物活性。

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  • 来源
    《Chemistry: A European journal》 |2019年第72期|共6页
  • 作者

    Qianyu Yu; Xiaoteng Ma; Yingze Liu; Hanying Zhao;

  • 作者单位

    Key Laboratory of Functional Polymer Materials Ministry of Education College of Chemistry Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) Nankai University Tianjin 300071 (P.R. China);

    Key Laboratory of Functional Polymer Materials Ministry of Education College of Chemistry Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) Nankai University Tianjin 300071 (P.R. China);

    Key Laboratory of Functional Polymer Materials Ministry of Education College of Chemistry Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) Nankai University Tianjin 300071 (P.R. China);

    Key Laboratory of Functional Polymer Materials Ministry of Education College of Chemistry Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) Nankai University Tianjin 300071 (P.R. China);

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 应用化学;
  • 关键词

    biomineralization; calcium; enzymes; gels; proteins;

    机译：生物碳化;钙;酶;凝胶;蛋白质;

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