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首页> 外文期刊>Chemistry: A European journal >Photoluminescent Peptide-Based Nanostructures as FRET Donor for Fluorophore Dye
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Photoluminescent Peptide-Based Nanostructures as FRET Donor for Fluorophore Dye

机译：基于光致发光的肽的纳米结构作为荧光团染料的褶皱供体

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摘要

A great interest has been recently generated by the discovery that peptide-based nanostructures (NSs) endowed with cross-β structure may show interesting photoluminescent (PL) properties. It was shown that NSs formed by PEGylated hexaphenylalanine (PEG_8-F_6, PEG=polyethylene glycol) are able to emit at 460 nm when excited at 370 or 410 nm. Here, the possibility to transfer the fluorescence of these PEG_8-F_6-based NSs by foster resonance electron transfer (FRET) phenomenon to a fluorescent dye was explored. To achieve this aim, the 4-chloro-7-nitrobenzofurazan (NBD) dye was encapsulated in these NSs. Structural data in solution and in solid state, obtained by a variety of techniques (circular dichroism, Fourier-transform infrared spectroscopy, wide-angle X-ray scattering, and small-angle X-ray scattering), indicated that the organization of the peptide spine of PEG_8-F_6 NS, which consists of anti-parallel β-sheets separated by a dry interface made of interacting phenylalanine side chains, was maintained upon NBD encapsulation. The spectroscopic characterization of these NSs clearly showed a redshift of the emission fluorescence peak both in solution and in solid state. This shift from 460 to 530 nm indicated that a FRET phenomenon from the peptide-based to the fluorophore- encapsulated NS occurred. FRET could also be detected in the PEG_8-F_6 conjugate, in which the NBD was covalently bound to the amine of the compound. On the basis of these results, it is suggested that the red-shift of the intrinsic PL of NSs may be exploited in the bio-imaging field.
机译：最近发现了一种令人兴趣的兴趣，发现赋予β结构的肽的纳米结构（NSS）可以显示有趣的光致发光（PL）性质。结果表明，当在370或410nm激发时，通过聚乙烯化己类氧丙氨酸（PEG_8-F_6，PEG =聚乙二醇）形成的NSS能够以460nm发射。这里，探讨了将基于PEG_8-F_6的NSS的荧光通过促进共振电子转移（FRET）现象转移到荧光染料的可能性。为了实现这一目标，将4-氯-7-硝基苯脲（NBD）染料包封在这些NSS中。通过各种技术（圆形二色性，傅立叶变换红外光谱，广角X射线散射和小角度X射线散射）获得的结构数据和固态。表明肽的组织PEG_8-F_6NS的脊柱，其由由苯丙氨酸侧链的干燥界面分离的抗平行β-薄片，在NBD包封时保持。这些NSS的光谱表征清楚地显示了溶液和固态的发射荧光峰的红移。从460到530 nm的这种偏移表明，从肽的基于荧光团的ns的褶皱现象发生。在PEG_8-F_6缀合物中也可以检测到FRET，其中NBD与化合物的胺共价结合。在这些结果的基础上，建议在生物成像场中利用NSS的内在PL的红转。

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  • 来源
    《Chemistry: A European journal》 |2017年第36期|共8页
  • 作者

    Carlo Diaferia; Teresa Sibillano; Cinzia Giannini; Valentina Roviello; Luigi Vitagliano; Giancarlo Morelli; Antonella Accardo;

  • 作者单位

    Department of Pharmacy Research Centre on Bioactive Peptides (CIRPeB) University of Naples “Federico II” and DFM Scarl Via Mezzocannone 16 80134 Naples (Italy);

    Institute of Crystallography (IC) CNR Via Amendola 122 70126 Bari (Italy);

    Institute of Crystallography (IC) CNR Via Amendola 122 70126 Bari (Italy);

    Analytical Chemistry for the Environment and CeSMA (Centro Servizi Metereologici Avanzati) University of Naples “Federico II” Corso Nicolangelo Protopisani 80146 Naples (Italy);

    Institute of Biostructures and Bioimaging (IBB) CNR Via Mezzocannone 16 80134 Naples (Italy);

    Department of Pharmacy Research Centre on Bioactive Peptides (CIRPeB) University of Naples “Federico II” and DFM Scarl Via Mezzocannone 16 80134 Naples (Italy);

    Department of Pharmacy Research Centre on Bioactive Peptides (CIRPeB) University of Naples “Federico II” and DFM Scarl Via Mezzocannone 16 80134 Naples (Italy);

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 应用化学;
  • 关键词

    cross-beta nanostructures; FRET; peptides; photoluminescence; polyphenylalanines;

    机译：十字β纳米结构;褶皱;肽;光致发光;聚苯甲苯胺;

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