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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >A modified protocol for total RNA isolation from different oil palm (Elaeis guineensis) tissues using cetyltrimethylammonium bromide
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A modified protocol for total RNA isolation from different oil palm (Elaeis guineensis) tissues using cetyltrimethylammonium bromide

机译：使用甲酰基三甲基铵溴来自不同油棕（ELAEISGuineensis）组织的RNA分离的改性方案

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摘要

Extraction of high-quality RNA from oil palm tissues is challenging due to the presence of polysaccharides, polyphenols and other complexes that co-precipitate with RNA. Therefore, isolation of high-quality RNA from oil palm is challenging due to the presence of varying amounts of these constituents in diverse tissues. This communication describes a modified RNA extraction protocol based on the cetyltrimethylammonium bromide (CTAB) method which is useful for extracting high-quality RNA from different oil palm tissues. Total RNA isolation using a modified CTAB protocol was compared with two different methods, a conventional TRIzol method and the method for RNA isolation from palms (MRIP). Both methods were useful for isolating RNA from leaf tissues; however, they were not effective in isolating RNA from other tissues. The current protocol based on a modified CTAB method was efficient in isolating high-quality total RNA from oil palm fruit tissues, including mesocarp and endosperm, stem, root and flower tissues. This modified CTAB-based protocol gave approximately 20-30 mu g of total RNA from 150 mg of tissue within 5-6 h.
机译：由于多糖，多酚和其他与RNA共沉淀的络合物存在，来自油棕组织的高质量RNA的提取是挑战性的。因此，由于在不同组织中存在不同量的这些成分，来自油棕的高质量RNA的分离是挑战性的。该通信描述了基于西甲基三甲基溴化铵（CTAB）方法的改性RNA提取方案，其可用于从不同的油棕榈组织中提取高质量的RNA。将使用修饰的CTAB方案的总RNA分离与两种不同的方法进行比较，常规Trizol方法和来自棕榈树（MRIP）的RNA分离的方法。两种方法可用于分离叶组织中的RNA;然而，它们在将来自其他组织中的RNA分离而无效。基于改性CTAB方法的当前方案有效地分离来自油棕榈果组织的高质量总RNA，包括Mesocarp和胚乳，茎，根和花组织。这种改进的CTAB基方案在5-6小时内从150mg组织中得到大约20-30μg的总RNA。

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  • 作者

    Qadri Rashad; Iqbal Amjad; Wu Yi; Li Jing; Nisar Numrah; Azam Muhammad; Yang Yaodong;

  • 作者单位

    Chinese Acad Trop Agr Sci Hainan Key Lab Trop Oil Crops Biol Coconut Res Inst Wenchang 571339 Hainan Peoples R China;

    Chinese Acad Trop Agr Sci Hainan Key Lab Trop Oil Crops Biol Coconut Res Inst Wenchang 571339 Hainan Peoples R China;

    Chinese Acad Trop Agr Sci Hainan Key Lab Trop Oil Crops Biol Coconut Res Inst Wenchang 571339 Hainan Peoples R China;

    Chinese Acad Trop Agr Sci Hainan Key Lab Trop Oil Crops Biol Coconut Res Inst Wenchang 571339 Hainan Peoples R China;

    Women Univ Lahore Lahore Coll Dept Environm Sci Lahore 54600 Pakistan;

    Univ Agr Faisalabad Inst Hort Sci Faisalabad Pakistan;

    Chinese Acad Trop Agr Sci Hainan Key Lab Trop Oil Crops Biol Coconut Res Inst Wenchang 571339 Hainan Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 自然科学现状及发展;
  • 关键词

    CTAB method; gene expression; oil palm; RNA extraction;

    机译：CTAB方法;基因表达;油棕;RNA提取;

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