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首页> 外文期刊>Advances in Experimental Medicine and Biology >MicroRNA-9 and Cell Proliferation in Lipopolysaccharide and Dexamethasone-Treated Naive and Desialylated A549 Cells Grown in Cigarette Smoke Conditioned Medium
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MicroRNA-9 and Cell Proliferation in Lipopolysaccharide and Dexamethasone-Treated Naive and Desialylated A549 Cells Grown in Cigarette Smoke Conditioned Medium

机译：MicroRNA-9和脂多糖中的细胞增殖和烟雾烟雾条件培养基中生长的脂多糖和地塞米松治疗的幼稚和脱裂化的A549细胞

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In this study we assessed microRNA-9 (miR-9) levels (RT-PCR) and cell proliferation (flow cytometry) in naive and desialylated human alveolar epithelial cells (A549 cells), grown for 24 h in cigarette smoke-conditioned medium. Cells were additionally treated with lipopolysaccharide (LPS) and/or dexamethasone. Proliferation positively correlated with miR-9 levels in both naive and desialylated cells. Cigarette smoke decreased miR-9 levels in both cell types by about three-fold but there was no significant correlation between both parameters. Dexamethasone was without substantial effect on cigarette smoke-induced changes in proliferation of naive cells, but some normalization was observed in desialylated cells. Dexamethasone increased miR-9 levels in both cell types grown in cigarette smoke-medium but the effect was stronger in desialylated cells. LPS increased cell proliferation and miR-9 by more than six-fold only in naive cells, while correlation coefficient for both parameters in cigarette smoke-LPS group was 0.41. Herein we identify miR-9 as the cigarette smoke (decrease) and LPS-responsive but dexamethasone-unresponsive microRNA. It is possible that increased miR-9 levels in naive A549 cells treated with LPS may be related to the activation of Toll-like receptor 4. Moreover, differences in cell response (both miR-9 and proliferation) to dexamethasone in naive and desialylated cells may point to non-genomic dexamethasone effects.
机译：在本研究中，我们评估了幼稚和脱粘的人肺泡上皮细胞（A549细胞）中的MicroRNA-9（MiR-9）水平（RT-PCR）和细胞增殖（流式细胞术），在香烟烟雾调节培养基中生长24小时。用脂多糖（LPS）和/或地塞米松另外处理细胞。扩散与幼稚和脱脂细胞中的miR-9水平正相关。卷烟烟雾在两种细胞类型中减少miR-9水平约3倍，但两个参数之间没有显着相关性。地塞米松对烟烟引起的幼稚细胞增殖的变化没有大量影响，但在脱胶细胞中观察到一些标准化。地塞米松在香烟中生长的细胞类型中增加了miR-9水平，但效果在脱胶细胞中较强。 LPS仅在幼稚细胞中增加细胞增殖和miR-9超过六倍以上，而香烟烟雾LPS组中的参数的相关系数为0.41。在此，我们将MiR-9鉴定为香烟烟雾（减少）和LPS响应但地塞米松的微小RNA。用LPS处理的幼稚A549细胞中的miR-9水平的增加可能与Toll样受体4的激活有关。此外，细胞反应（miR-9和增殖）的差异在幼稚和脱胶细胞中的地塞米松可以指向非基因组塞米松效应。

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