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首页> 外文期刊>ACS applied materials & interfaces >The Protein Mat(ters)—Revealing the Biologically Relevant Mechanical Contribution of Collagen- and Fibronectin-Coated Micropatterns
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The Protein Mat(ters)—Revealing the Biologically Relevant Mechanical Contribution of Collagen- and Fibronectin-Coated Micropatterns

机译:蛋白质垫(TERS) - 抗于胶原和纤连蛋白涂层微图案的生物相关机械贡献

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摘要

Understanding cell–material interactions requires accurate characterization of the substrate mechanics, which are generally measured by indentation-type atomic force microscopy. To facilitate cell–substrate interaction, model extracellular matrix coatings are used although their tensile mechanical properties are generally unknown. In this study, beyond standard compressive stiffness estimation, we performed a novel tensile mechanical characterization of collagen- and fibronectin-micropatterned polyacrylamide hydrogels. We further demonstrate the impact of the protein mat on the tensile stiffness characterization of adherent cells. To our knowledge, our study is the first to uncover direction-dependent mechanical behavior of the protein coatings and to demonstrate that it affects cellular response relative to substrate mechanics.
机译:理解细胞 - 材料相互作用需要精确表征基材的基础力学,其通常通过压痕型原子力显微镜测量。 为了促进细胞基板相互作用,使用模型的细胞外基质涂层,尽管它们的拉伸机械性能通常是未知的。 在本研究中,除标准压缩刚度估计之外,我们进行了一种新型胶原和纤连蛋白微型聚丙烯酰胺水凝胶的抗拉机械表征。 我们进一步证明了蛋白质垫对粘附细胞的拉伸刚度表征的影响。 据我们所知,我们的研究是第一个揭示蛋白质涂料的方向依赖性机械行为,并证明它影响相对于基板力学的细胞响应。

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