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首页> 外文期刊>Angewandte Botanik: Zeitschrift der Vereinigung fur Angewandte Botanik >Effect of exogenous growth regulators on organogenesis of tissue culturesof ageratina riparia regel (asteraceae) [German]
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Effect of exogenous growth regulators on organogenesis of tissue culturesof ageratina riparia regel (asteraceae) [German]

机译：外源生长调节剂对河南紫花苜蓿（菊科）组织培养器官发生的影响[德语]

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摘要

Investigation was undertaken to achieve rapid multiplication and improvement of Ageratina, riparia (Asteraceae)by plant regeneration from callus. Tissue cultures of A, riparia derived from leaf-, stem-and root tissues were cultivated on modified MURASHIGE and SKOOG, 1962 and ABou-MANDOUR, 1977 media. Growth of all callus cultures was stimulated by the phytohormones applied (KIN, BAP, IAA, NAA, 2,4-D). Calli derived from leaves exhibited the strangest response to hormone treatment, followed by calli from stems and roots. Highest growth rates were obtained on MS with 0,3 mg I-1 KIN! and 2 mg l(-1) IAA, and AM with 2 mg l(-1) of KIN, IAA and BAP. Intact plants were regenerated from calli derived from both leaves and roots. When roots of these regenerates were cultivated on MS with 0,3 mg.l(-1) KIN and 4 mg.l(-1) NAA they became green if originated from leaf callus or grey white with many roothairs if originated from root callus. With MS + 2 mg.l(-1) BAP these roots again formed calli. On MS with high concentrations of 2,4-D (4 - 8 mg.l(-1))root regeneration was suppressed Micropropagated plants were successfully transferred to soil.
机译：进行了研究，以通过从愈伤组织中再生植物来实现快速繁殖和改善河豚草（菊科）。在改良的MURASHIGE和SKOOG（1962年）和ABou-MANDOUR（1977年）培养基上培养源自叶，茎和根组织的A，河豚草的组织培养物。施用的植物激素（KIN，BAP，IAA，NAA，2,4-D）刺激所有愈伤组织培养物的生长。叶片的愈伤组织对激素处理表现出最奇怪的反应，其次是茎和根。在0.3 mg I-1 KIN的MS上获得最高的生长速率！和2 mg l（-1）IAA，以及AM与2 mg l（-1）KIN，IAA和BAP。完整的植物从叶片和根的愈伤组织中再生。当这些再生的根在含有0.3 mg.l（-1）KIN和4 mg.l（-1）NAA的MS上培养时，如果它们起源于叶愈伤组织，则它们变为绿色;如果起源于根愈伤组织，则它们变为灰白色，具有许多根毛。 。用MS + 2 mg.l（-1）BAP，这些根再次形成愈伤组织。在高浓度的2,4-D（4-8 mg.l（-1））的MS上，根再生受到抑制，微繁殖的植物成功转移到土壤中。

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