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首页> 外文期刊>ACS applied materials & interfaces >Fluorescent TPA@GQDs Probe for Sensitive Assay and Quantitative Imaging of Hydroxyl Radicals in Living Cells
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Fluorescent TPA@GQDs Probe for Sensitive Assay and Quantitative Imaging of Hydroxyl Radicals in Living Cells

机译:用于敏感测定的荧光TPA @ GQDS探针和活细胞中羟基自由基的定量成像

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摘要

A fluorescent probe TPA@GQDs is fabricated by the conjugation of terephthalic acid (TPA) on the surface of graphene quantum dots (GQDs). The TPA@GQDs probe not only has favorable dispersibility but also exhibits excellent fluorescence stability over a wide pH range and high ionic strength and favorable antiphotobleaching ability. The great fluorescence enhancement of TPA@GQDs induced by the reaction between TPA and hydroxyl radicals makes the TPA@GQDs a powerful probe for the sensitive assay of hydroxyl radicals, giving rise to a low detection limit down to 12 nmol L-1. Meanwhile, the obtained fluorescent TPA@GQDs probe shows low cytotoxicity and favorable biocompatibility. Its potential in bioimaging is demonstrated by the quantitative fluorescent imaging of hydroxyl radicals in living HeLa cells under different circumstances, which enables the opportunities to study hydroxyl radicals dynainics in living cells.
机译:通过对苯二甲酸(TPA)在石墨烯量子点(GQD)表面上的缀合来制造荧光探针TPA @ GQD。 TPA @ GQDS探针不仅具有良好的分散性,而且还在宽的pH范围内具有优异的荧光稳定性和高离子强度和有利的抗邻晶晶体能力。 TPA和羟基之间反应诱导的TPA @ GQD的大荧光增强使TPA @ GQDS为羟基自由基的敏感测定产生强大的探针,从而导致低于12nmol L-1的低检测限。 同时,所得荧光TPA @ GQDS探针显示出低细胞毒性和有利的生物相容性。 在不同情况下,活性HeLa细胞中羟基自由基的定量荧光成像证明了其在生物模仿中的潜力,这使得能够研究活细胞中的羟基自由基染发管。

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