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首页> 外文期刊>American Journal of Physiology >Dynamic functional contribution of the water channel AQP5 to the water permeability of peripheral lens fiber cells
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Dynamic functional contribution of the water channel AQP5 to the water permeability of peripheral lens fiber cells

机译：水通道AQP5对外周透镜纤维细胞的渗透性的动态功能贡献

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摘要

Although the functionality of the lens water channels aquaporin 1 (AQP1; epithelium) and AQPO (fiber cells) is well established, less is known about the role of AQP5 in the lens. Since in other tissues AQP5 functions as a regulated water channel with a water permeability (P_(h_2o)) some 20 times higher than AQPO, AQP5 could function to modulate P_(h_2o) in lens fiber cells. To test this possibility, a fluorescence dye dilution assay was used to calculate the relative P_(h_2o) of epithelial cells and fiber membrane vesicles isolated from either the mouse or rat lens, in the absence and presence of HgCl_2, an inhibitor of AQP1 and AQP5. Immunolabeling of lens sections and fiber membrane vesicles from mouse and rat lenses revealed differences in the subcellular distributions of AQP5 in the outer cortex between species, with AQP5 being predominantly membranous in the mouse but predominantly cytoplas-mic in the rat. In contrast, AQPO labeling was always membranous in both species. This species-specific heterogeneity in AQP5 membrane localization was mirrored in measurements of P_(h_2o), with only fiber membrane vesicles isolated from the mouse lens, exhibiting a significant Hg~(2+)-sensitive contribution to P_(h_2o). When rat lenses were first organ cultured, immunolabeling revealed an insertion of AQP5 into cortical fiber cells, and a significant increase in Hg~(2+)-sensitive P_(h_2o) was detected in membrane vesicles. Our results show that AQP5 forms functional water channels in the rodent lens, and they suggest that dynamic membrane insertion of AQP5 may regulate water fluxes in the lens by modulating P_(h_2o) in the outer cortex.
机译：尽管镜片水通道Aquaporin 1（AQP1;上皮）和AQPO（纤维细胞）的功能是很好的，但是关于AQP5在镜片中的作用较少。由于在其他组织中，AQP5用作透水性的调节水通道（P_（H_2O））比aQPO高约20倍，AQP5可以起到调节透镜光纤细胞中的P_（H_2O）。为了测试这种可能性，使用荧光染料稀释测定来计算上皮细胞和从小鼠或大鼠镜片中分离的上皮细胞和纤维膜囊泡的相对p_（H_2O），在HgCl_2的不存在和存在下，AQP1和AQP5的抑制剂。来自小鼠和大鼠镜片的透镜部分和纤维膜囊泡的免疫标签揭示了物种之间的外皮中AQP5的亚细胞分布的差异，AQP5主要在小鼠中膜，但大鼠中主要是细胞质-MIC。相反，AQPO标记在这两个物种中总是膜。在P_（H_2O）的测量中反映了AQP5膜定位中的该物种特异性异质性，只有从小鼠镜片中分离的纤维膜囊泡，表现出对P_（H_2O）的显着的HG〜（2 +）敏感贡献。当大鼠镜片是培养的第一个器官时，免疫标签显示出AQP5插入皮质纤维细胞，并且在膜囊泡中检测到Hg〜（2 +）敏感的P_（H_2O）的显着增加。我们的结果表明，AQP5在啮齿动物镜片中形成功能水通道，并表明AQP5的动态膜插入可以通过调节外皮中的P_（H_2O）调节透镜中的水量。

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