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首页> 外文期刊>American Journal of Physiology >First evidence of nocturnin in fish: two isoforms in goldfish differentially regulated by feeding
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First evidence of nocturnin in fish: two isoforms in goldfish differentially regulated by feeding

机译：FISH中夜间素的第一个证据：通过喂养差异调节的金鱼两种同种型

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Nocturnin (NOC) is a unique deadenylase with robust rhythmic expression involved in the regulation of metabolic processes in mammals. Currently, the possible presence of NOC in fish is unknown. This report aimed to identify NOC in a fish model, the goldfish (Carassius auratus), and to study the possible regulation of its expression by feeding. Two partial-length cDNAs of 293 and 223 bp, named noctumin-a (noc-a) and nocturnin-b (noc-b), were identified and found to be highly conserved among vertebrates. Both mRNAs show a similar widespread distribution in central and peripheral tissues, with higher levels detected for noc-a compared with noc-b. The periprandial expression profile revealed that noc-a mRNAs rise sharply after a meal in hypothalamus, intestinal bulb, and liver, whereas almost no changes were observed for noc-b. Food deprivation was found to exert opposite effects on the expression of both NOCs (generally inhibitory for noc-a, and stimulatory for noc-b) in the three mentioned tissues. A single meal after a 48-h food deprivation period reversed (totally or partially) the fasting-induced decreases in noc-a transcripts in all studied tissues and the increases in noc-b expression in the intestinal bulb. Together, this study offers the first report of NOC in fish and shows a high dependence of its expression on feeding and nutritional status. The differential responses to feeding of the two NOCs raise the possibility that they might be underlying different physiological mechanisms (e.g., food intake, lipid mobilization, energy homeostasis) in fish.
机译：Nocturnin（Noc）是一种独特的硬烯基酶，具有较强的节奏表达，参与了哺乳动物中代谢过程的调节。目前，鱼中可能存在的鱼类是未知的。本报告旨在在鱼类模型，金鱼（Carassius auratus）中识别NOC，并通过饲养来研究其表达的可能调节。鉴定了293和223bp的两种部分长度CDNA，名为NOCTUME-A（NOC-A）和NOCTRNIN-B（NOC-B），并发现脊椎动物中的高度保守。两个MRNA在中央和外周组织中显示出类似的广泛分布，与NOC-B相比，NOC-A检测到更高的水平。围裙表达型谱显示，在下丘脑，肠泡和肝脏膳食后，NOC-A MRNA急剧上升，而NOC-B几乎没有观察到任何变化。发现食品剥夺在三种上述组织中对NOCs（通常抑制的NOC-A和NOC-B）的表达施加相反的影响。在48小时食物剥夺期后的单粉（完全或部分）逆转（完全或部分），NOC-A转录物中的禁食诱导的诱导的组织和肠泡中NOC-B表达的增加。这项研究在一起，提供了FISH中NOC的第一份报告，并表现出对饲养和营养状况的表达的高度依赖性。对两国NOC的喂养的差异反应提出了它们在鱼中可能潜在的不同生理机制（例如食物摄入，脂肪动员，能源稳态）。

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