A Photolabile Semiconducting Polymer Nanotransducer for Near‐Infrared Regulation of CRISPR/Cas9 Gene Editing

Lyu Yan; He Shasha; Li Jingchao; Jiang Yuyan; Sun He; Miao Yansong; Pu Kanyi

摘要

Abstract > Noninvasive regulation of CRISPR/Cas9 gene editing is conducive to understanding of gene function and development of gene therapy; however, it remains challenging. Herein, a photolabile semiconducting polymer nanotransducer (pSPN) is synthesized to act as the gene vector to deliver CRISPR/Cas9 plasmids into cells and also as the photoregulator to remotely activate gene editing. pSPN comprises a 1 O <sub>2</sub> ‐generating backbone grafted with polyethylenimine brushes through 1 O <sub>2</sub> ‐cleavable linkers. NIR photoirradiation spontaneously triggers the cleavage of gene vectors from pSPN, resulting in the release of CRISPR/Cas9 plasmids and subsequently initiating gene editing. This system affords 15‐ and 1.8‐fold enhancement in repaired gene expression relative to the nonirradiated controls in living cells and mice, respectively. As this approach does not require any specific modifications on biomolecular components, pSPN represents the first generic nanotransducer for in?vivo regulation of CRISPR/Cas9 gene editing. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> > Frespr / Cas9基因编辑的非侵入性调节有利于理解基因函数和基因治疗的发展;但是，它仍然具有挑战性。这里，合成光型半导体聚合物纳米转移器（PSPN）以充当基因载体，以将CRISPR / CAS9质粒递送到细胞中，并作为光学激活基因编辑的光调用器。 PSPN包括A. 1 </ sup> O. <sub> 2 </ sub> - 通过聚乙胺刷接枝的根骨干 1 </ sup> O. <sub> 2 </ sub> - 可生活的接头。 NIR PhotoRaradiation自发地触发来自PSPN的基因载体的切割，导致CRISPR / CAS9质粒的释放和随后启动基因编辑。该系统分别在相对于活细胞和小鼠中的非辐射对照中的修复基因表达中提供15-和1.8倍的增强。由于这种方法不需要对生物分子组分的任何特异性修饰，PSPN代表第一通用纳米转移器，用于体内调节CRISPR / CAS9基因编辑。 </ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-19011/'>《Angewandte Chemie》</a> <b style="margin: 0 2px;">|</b><span>2019年第50期</span><b style="margin: 0 2px;">|</b><span>共5页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lyu Yan&option=202" target="_blank" rel="nofollow">Lyu Yan;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=He Shasha&option=202" target="_blank" rel="nofollow">He Shasha;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Li Jingchao&option=202" target="_blank" rel="nofollow">Li Jingchao;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Jiang Yuyan&option=202" target="_blank" rel="nofollow">Jiang Yuyan;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sun He&option=202" target="_blank" rel="nofollow">Sun He;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Miao Yansong&option=202" target="_blank" rel="nofollow">Miao Yansong;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Pu Kanyi&option=202" target="_blank" rel="nofollow">Pu Kanyi;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>School of Chemical and Biomedical EngineeringNanyang Technological University70 Nanyang Drive Singapore 637457 Singapore;</p> <p>School of Chemical and Biomedical EngineeringNanyang Technological University70 Nanyang Drive Singapore 637457 Singapore;</p> <p>School of Chemical and Biomedical EngineeringNanyang Technological University70 Nanyang Drive Singapore 637457 Singapore;</p> <p>School of Chemical and Biomedical EngineeringNanyang Technological University70 Nanyang Drive Singapore 637457 Singapore;</p> <p>School of Biological ScienceNanyang Technological University60 Nanyang Drive Singapore 637551 Singapore;</p> <p>School of Chemical and Biomedical EngineeringNanyang Technological University70 Nanyang Drive Singapore 637457 Singapore;</p> <p>School of Chemical and Biomedical EngineeringNanyang Technological University70 Nanyang Drive Singapore 637457 Singapore;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/1188.html" title="应用化学">应用化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=CRISPR/Cas9&option=203" rel="nofollow">CRISPR/Cas9;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=gene editing&option=203" rel="nofollow">gene editing;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=nanotechnology&option=203" rel="nofollow">nanotechnology;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=photoregulation&option=203" rel="nofollow">photoregulation;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=semiconducting polymers&option=203" rel="nofollow">semiconducting polymers;</a> </p> <div class="translation"> 机译：CRISPR / CAS9;基因编辑;纳米技术;光调节;半导体聚合物; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704028163391.html">A Photolabile Semiconducting Polymer Nanotransducer for Near‐Infrared Regulation of CRISPR/Cas9 Gene Editing</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lyu Yan&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Lyu Yan,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=He Shasha&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">He Shasha,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Li Jingchao&option=202" target="_blank" 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