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首页> 外文期刊>Applied Microbiology and Biotechnology >Dps protein is related to resistance of Mycobacterium abscessus subsp. massiliense against stressful conditions
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Dps protein is related to resistance of Mycobacterium abscessus subsp. massiliense against stressful conditions

机译：DPS蛋白质与分枝杆菌脓肿患者的抗性有关。 针对压力条件的屠杀

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Mycobacterium abscessus subsp. massiliense (Mycma) belongs to the Mycobacterium abscessus complex and is a rapidly growing non-tuberculous mycobacterium. The chronic pulmonary, skin, and soft tissue infections that it causes may be difficult to treat due to its intrinsic resistance to the commonly used antimicrobial drugs, making it a serious world public health problem. Iron is an essential nutrient for the growth of microorganisms; nonetheless, it can be toxic when in excess. Thus, bacteria require an iron homeostasis mechanism to succeed in different environments. DNA-binding proteins from starved cells (Dps) are miniferritins with the property to act as additional iron storage proteins but also can bind to DNA, protecting it against hydroxyl radical. Annotation of the Mycma genome revealed the gene mycma_03135 with 79% sequential identity when compared to MSMEG_3242 gene from M. smegmatis mc(2) 155, which codifies for a known Dps. Recombinant Dps from M. abscessus (rMaDps) was produced in Escherichia coli, purified in soluble form and shown to form high mass oligomers in solution with ferroxidase activity, DNA binding, and protection against damage. The expression of the mycma_03135 gene was induced during Mycma growth in the presence of hydrogen peroxide (H2O2). Additionally, the expression of rMaDps by E. coli conferred greater resistance to H2O2. Thus, this study is the first to identify and characterize a Dps from M. abscessus.
机译：分枝杆菌脓肿亚空间。 Massiliense（Mycma）属于脓肿复合物的分枝杆菌复合物，是一种快速生长的非结核分枝杆菌。由于其对常用抗菌药物的内在抗性，慢性肺，皮肤和软组织感染可能难以治疗，使其成为一个严重的世界公共卫生问题。铁是微生物生长的必要营养素;尽管如此，它可能在超出时毒性。因此，细菌需要铁稳态机制在不同的环境中取得成功。来自饥饿细胞（DPS）的DNA结合蛋白是小亚烷烃，其具有额外的铁储存蛋白，但也可以与DNA结合，保护它免受羟基。与来自M.Smogmatis MC（2）155的Mmmeg_3242基因相比，Mycma基因组的注释显示出具有79％的顺序同一性的基因mycma_03135，其编写了已知DPS。来自M.脓肿（RMADPS）的重组DPS在大肠杆菌中生产，以可溶性纯化，并显示在溶液中形成高质量低聚物，具有铁氧基酶活性，DNA结合和防止损伤。在过氧化氢（H2O2）的存在下诱导Mycma_03135基因的表达。另外，大肠杆菌的RMADPS的表达赋予了更大的对H 2 O 2的抗性。因此，本研究是第一个识别和表征来自脓肿的DPS。

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