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首页> 外文期刊>Applied Microbiology and Biotechnology >Leveraging nature's biomolecular designs in next-generation protein sequencing reagent development
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Leveraging nature's biomolecular designs in next-generation protein sequencing reagent development

机译：利用大自然在下一代蛋白质测序试剂发育中的生物分子设计

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Next-generation approaches for protein sequencing are now emerging that could have the potential to revolutionize the field in proteomics. One such sequencing method involves fluorescence-based imaging of immobilized peptides in which the N-terminal amino acid of a polypeptide is readout sequentially by a series of fluorescently labeled biomolecules. When selectively bound to a specific N-terminal amino acid, the NAAB (N-terminal amino acid binder) affinity reagent identifies the amino acid through its associated fluorescence tag. A key technical challenge in implementing this fluoro-sequencing approach is the need to develop NAAB affinity reagents with the high affinity and selectivity for specific N-terminal amino acids required for this biotechnology application. One approach to develop such a NAAB affinity reagent is to leverage naturally occurring biomolecules that bind amino acids and/or peptides. Here, we describe several candidate biomolecules that could be considered for this purpose and discuss the potential for developability of each.
机译：现在，蛋白质测序的下一代方法可以具有促进蛋白质组学中的田地的潜力。一种这样的测序方法涉及固定化肽的基于荧光的成像，其中通过一系列荧光标记的生物分子依次读出多肽的N-末端氨基酸。 When selectively bound to a specific N-terminal amino acid, the NAAB (N-terminal amino acid binder) affinity reagent identifies the amino acid through its associated fluorescence tag.实施该氟测序方法的关键技术挑战是需要使用该生物技术应用所需的特异性N-末端氨基酸具有高亲和力和选择性的Naab亲和试剂。一种发展这种Naab亲和试剂的方法是利用自然发生的生物分子，其结合氨基酸和/或肽。在这里，我们描述了几种可以考虑的候选生物分子，可以考虑此目的，并讨论每个的潜力。

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