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首页> 外文期刊>Applied Microbiology and Biotechnology >Development ofTomato bushy stunt virus-based vectors for fusion and non-fusion expression of heterologous proteins in an alternative hostNicotiana excelsiana
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Development ofTomato bushy stunt virus-based vectors for fusion and non-fusion expression of heterologous proteins in an alternative hostNicotiana excelsiana

机译：替代宿主尼替氏素蛋白酶蛋白蛋白质融合和非融合表达的基于摩尔族特技载体的发展

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Plant virus-based expression systems are an alternative expression platform for the production of clinically and industrially useful recombinant proteins. Nonetheless, due to a lack of viral vector with the commercial potentials, it is urgent to design and develop new, versatile, and efficient plant virus vectors. The genome ofTomato bushy stunt virus(TBSV) offers an attractive alternative to being modified as a vector for producing heterologous proteins in plants. Here, we developed a set of novel fusion and non-fusion TBSV-CP replacement vectors, which provide more flexible and efficient tools for expressing proteins of interest in plants. An alternative tobacco plant,Nicotiana excelsiana, was used in this study as a host for newly constructed TBSV vectors because the unwanted necrotic effects were reported on the commonly usedNicotiana benthamianahost associated with expression of TBSV-encoded P19 protein. The data showed that TBSV vectors caused a symptomless infection and overexpressed reporter gene inN. excelsianaleaves, demonstrating thatN. excelsianais an ideal host plant for TBSV-mediated heterologous gene expression.Moreover, a TBSV non-fusion vector, dAUG, shows the similar accumulation level of reporter proteins to that of TMV- and PVX-based vectors in side-by-side comparison and provides more flexible aspects than the previously developed TBSV vectors. Collectively, our newly developed TBSV expression system adds a new member to the family of plant viral expression vectors and meanwhile offers a flexible and highly effective approach for producing proteins of interest in plants.
机译：基于植物病毒的表达系统是用于在临床和工业上有用的重组蛋白的生产的替代表达平台。尽管如此，由于缺乏具有商业潜力的病毒载体，迫切需要设计和开发新的通用和高效的植物病毒载体。基因组·伯利·特技病毒（TBSV）提供了一种有吸引力的替代方案，被修饰为用于在植物中生产异源蛋白的载体。在这里，我们开发了一组新颖的融合和非融合TBSV-CP更换载体，其为表达植物植物的蛋白质提供更灵活和有效的工具。替代烟草植物，尼古拉替斯基替斯坦，在本研究中使用作为新构建的TBSV载体的宿主，因为在与TBSV编码的P19蛋白表达相关的常见使用者的弯曲作用上报告了不需要的坏死作用。该数据显示TBSV载体导致症状感染和过表达的报告总因宾馆。 excelsianaleaves，展示那个。 Excelsianais是TBSV介导的异源基因表达的理想宿主植物。ORE，TBSV非融合载体，DAG，在并排比较中显示了报告蛋白与TMV-和PVX基载体的类似累积水平。提供比以前开发的TBSV向量更灵活的方面。统称，我们的新开发的TBSV表达系统为植物病毒表达载体系列添加了新成员，同时提供了一种灵活且高效的方法，可以生产植物植物的蛋白质。

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