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首页> 外文期刊>Applied Microbiology and Biotechnology >Comparative proteomic analysis: SclR is importantly involved in carbohydrate metabolism in Aspergillus oryzae
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Comparative proteomic analysis: SclR is importantly involved in carbohydrate metabolism in Aspergillus oryzae

机译：比较蛋白质组学分析：SCLR主要参与Aspergillus Oryzae的碳水化合物代谢

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摘要

The helix-loop-helix (HLH) family of transcriptional factors is a key player in a wide range of developmental processes in organisms from mammals to microbes. We previously identified the bHLH transcription factor SclR in Aspergillus oryzae and found that the loss of SclR function led to significant phenotypic changes, such as rapid protein degradation and cell lysis in dextrin-polypeptone-yeast extract liquid medium. The result implied that SclR is potentially important in both traditional fermentative manufacturing and commercial enzyme production in A. oryzae because of its effect on growth. Therefore, this study presents a comparative assessment at the proteome level of the intracellular differences between an sclR-disrupted strain and a control strain using isobaric tandem mass tag (TMT) labeling for quantification. A total of 5447 proteins were identified, and 568 were differentially expressed proteins (DEPs). Of the DEPs, 251 proteins were increased by 1.5-fold, and 317 proteins were decreased by 1.5-fold in an sclR-disrupted strain compared to the control. The comparison of the quantitative TMT results revealed that SclR was mainly involved in carbon metabolism, especially carbohydrate metabolism. In addition, an enzyme profile by a semi-quantitative method (API-ZYM) indicated that three enzymes (beta-galactosidase, alpha-glucosidase, and alpha-mannosidase) were significantly less active in the a dagger sclR strain than in the control. Moreover, quantitative RT-PCR showed that the expression of certain genes was changed similarly to their corresponding proteins. These results suggested that a possible function of SclR during growth of A. oryzae is its important involvement in carbohydrate metabolism.
机译：Helix-Loop-Helix（HLH）的转录因子系列是哺乳动物对微生物的各种发育过程中的关键球员。我们以前鉴定了曲霉属植物中的BHLH转录因子SCLR，发现SCLR功能的损失导致了显着的表型变化，例如糊精 - 多肽 - 酵母萃取液中的快速蛋白质降解和细胞裂解。结果暗示，由于其对生长的影响，SCLR在传统的发酵制造和商业酶生产中可能是重要的。因此，本研究在使用同位串联质量标签（TMT）标记进行量化的SCLR破坏的菌株与对照菌株之间的细胞内差异的蛋白质组水平的比较评估。鉴定了总共5447个蛋白质，并且568例是差异表达蛋白质（DEPS）。在DEPS中，与对照相比，在SCLR破碎的菌株中，251个蛋白质增加了1.5倍，317个蛋白质在SCLR破裂的菌株中降低了1.5倍。定量TMT结果的比较显示，SCLR主要涉及碳代谢，尤其是碳水化合物代谢。另外，通过半定量方法（API-ZYM）的酶谱表明，在匕首SCLR应变中，三种酶（β-半乳糖苷酶，α-葡糖苷酶和α-甘氨酸酶）显着较低较低。此外，定量RT-PCR表明，某些基因的表达类似地与其相应的蛋白质相似。这些结果表明，SCLR在A或yzae生长期间的可能功能是其在碳水化合物代谢中的重要累及。

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