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首页> 外文期刊>Applied Microbiology and Biotechnology >ARTP mutation and genome shuffling of ABE fermentation symbiotic system for improvement of butanol production
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ARTP mutation and genome shuffling of ABE fermentation symbiotic system for improvement of butanol production

机译：ABE发酵共生体系改善丁醇产量的ARTP突变和基因组。

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Butanol is an ideal renewable biofuel which possesses superior fuel properties. Previously, butanol-producing symbiotic system TSH06 was isolated in our lab, with microoxygen tolerance ability. To boost butanol yield for large-scale industrial production, TSH06 was used as parental strain and subjected to atmospheric and room temperature plasma (ARTP) and four rounds of genome shuffling (GS). ARTP mutant and GS strain were co-cultured with facultative anaerobic Bacillus cereus TSH2 to form a symbiotic system with microoxygen tolerance, which was then subjected to fermentation. Relative messenger RNA (mRNA) level of key enzyme gene was measured by real-time PCR. The highest butanol titer of TS4-30 reached 15.63 g/L, which was 34% higher than TSH06 (12.19 g/L). Compared with parental strain, mRNA of acid-forming gene in TS4-30 decreased in acidogenesis phase, while solvent-forming gene increased in solventogenesis phase. This gene expression pattern was consistent with high butanol yield and low acid level in TS4-30. In summary, symbiotic system TS4-30 was obtained with butanol titer improvement and microoxygen tolerance.
机译：丁醇是一个理想的可再生生物燃料，具有卓越的燃料特性。以前，在我们的实验室中分离出丁醇产生的共生体系TSH06，具有微氧体公差能力。为了提高大规模工业生产的丁醇产量，TSH06被用作父母菌株，经受大气和室温等离子体（ARTP）和四轮基因组混洗（GS）。 ARTP突变体和GS菌株与兼性厌氧芽孢杆菌TSH2共培养，形成具有微氧体耐受的共生体系，然后进行发酵。通过实时PCR测量关键酶基因的相对信使RNA（mRNA）水平。 TS4-30的最高丁醇滴度达到15.63克/升，比TSH06高出34％（12.19克/升）。与父母菌株相比，TS4-30中的酸形成基因的mRNA在呈现相中降低，而溶剂形成基因在溶剂发生相中增加。该基因表达模式与TS4-30中高丁醇产率和低酸水平一致。总之，用丁醇滴度和微氧体耐受获得共生系统TS4-30。

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