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首页> 外文期刊>Applied Microbiology and Biotechnology >Study of ChiR function in Serratia marcescens and its application for improving 2,3-butanediol from crystal chitin
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Study of ChiR function in Serratia marcescens and its application for improving 2,3-butanediol from crystal chitin

机译：Serratia Marcescens的Chir功能及其改进2,3-丁二醇从Crysty Chitin的应用研究

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摘要

Microbial utilization of chitin, a potential renewable biomass feedstock, is being pursued as a means of developing novel consolidated bioprocessing for the production of chemicals. Serratia marcescens is a gram-negative bacterium that is known for its chitinolytic capability and as a native 2,3-butanediol producer. In S. marcescens, ChiR has been suggested to be a positive regulator of chitinase production. In this study, we aim to understand the effect of ChiR in regulating nine chitinase-related genes in S. marcescens Db11 and demonstrate manipulation of chiR as a useful and efficient genetic target to enhance chitin utilization. First, a chiR overexpression (chiROE) strain and a chiR deletion (Delta chiR) strain were generated and characterized in terms of cellular growth, chitinase activity, and total secreted protein. Compared to the wild-type Db11 strain, the S. marcescens chiROE strain showed an increase in chitinase activity (2.14- to 6.31-fold increase). Increased transcriptional expression of chitinase-related genes was measured using real-time PCR, showing 2.12- to 10.93-fold increases. The S. marcescens Delta chiR strain showed decreases in chitinase activity (4.5- to 25-fold decrease), confirming ChiR's role as a positive regulator of chitinase expression. Finally, chiR overexpression was investigated as a means of increasing biochemical production (2,3-butanediol) from crystal chitin. The chiROE strain produced 1.13 +/- 0.08 g/L 2,3-butanediol from 2% crystal chitin, a 2.83-fold improvement from the wild-type strain, indicating ChiR is an important and useful genetic engineering target for enhancing chitin utilization in S. marcescens.
机译：依肉蛋白的微生物利用是一种潜在的可再生生物量原料，作为开发新型综合生物加工的手段，用于生产化学品。 Serratia Marcescens是一种革兰氏阴性细菌，其含量为其几丁醇溶解能力和天然2,3-丁二醇生产商。在S. Marcescens，已经建议Chir是一丁酶生产的正稳压因素。在这项研究中，我们旨在了解Chir在S.Marcescens DB11中调节九丁质酶相关基因的效果，并证明Chir作为一种有用和有效的遗传靶来提高甲藻利用率。首先，在细胞生长，几丁质酶活性和总分泌蛋白质方面产生并表征肾过度表达（Chiroe）菌株和Chir缺失（Delta Chir）菌株。与野生型DB11菌株相比，S.Marcescens Chirooe菌株显示逐蛋白酶活性增加（增加2.14-6.31倍）。使用实时PCR测量几丁质酶相关基因的转录表达增加，显示出2.12-至10.93倍。 S.Marcescens Delta Chir菌株表现出几丁质酶活性（减少4.5至25倍），确认Chir作为几丁质酶表达的正调节剂的作用。最后，研究了Chir过度表达作为从晶壳含量增加生化生产（2,3-丁二醇）的手段。从2％晶壳的啁啾菌株产生1.13 +/- 0.08g / l 2,3-丁二醇，从野生型菌株的改善2.83倍，表明潮是一种重要而有用的基因工程靶标，用于提高甲壳素利用S. Marcescens。

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