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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Delivery of Apoptosis-inducing Piperine to Triple-negative Breast Cancer Cells via Co-polymeric Nanoparticles
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Delivery of Apoptosis-inducing Piperine to Triple-negative Breast Cancer Cells via Co-polymeric Nanoparticles

机译：通过共聚物纳米颗粒递送凋亡诱导的哌啶至三阴性乳腺癌细胞

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Background/Aim: Piperine, a major alkaloid of the fruit of black pepper plants, selectively inhibits the growth of triple-negative breast cancer cells but its lipophilicity restricts possible clinical application. This study therefore determined the feasibility of encapsulating piperine in nanoparticles (NPs) to increase its solubility in an aqueous environment. Materials and Methods: Piperine-loaded biodegradable methoxy poly(ethylene glycol)poly(lactic-co-glycolic) acid copolymer-based NPs were produced by single emulsion solvent extraction and thin-film hydration. Growth and viability of triple-negative breast cancer (TNBC) cells were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and Annexin-V-FLUOS/propidium iodide staining, respectively. Results: Thin-film hydration was superior to single emulsion solvent extraction, yielding piperine-loaded NPs with an average size of 50 nm. Piperine-loaded NPs inhibited TNBC cell growth and induced apoptosis while sparing normal fibroblasts. Conclusion: It is feasible to deliver a cytotoxic concentration of piperine to TNBC cells via NPs with the potential for improved bioavailability and solubility in biological fluids.
机译：背景/目的：哌啶，黑胡椒植物果实的主要生物碱，有选择性地抑制三阴性乳腺癌细胞的生长，但其亲脂性限制了可能的临床应用。因此，该研究确定了包封哌啶在纳米颗粒（NPS）中的可行性，以增加其在水性环境中的溶解度。材料和方法：通过单一乳液溶剂萃取和薄膜水合制备哌啶加载的可生物降解的甲氧基聚（乙二醇）聚（乳酸二乙醇酸）酸共聚物基NP。三阴性乳腺癌（TNBC）细胞的生长和活力由3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴（MTT）测定和碘化吡啶胺染色和碘化物染色测定， 分别。结果：薄膜水合优于单一乳液溶剂萃取，产哌啶加载的NP，平均尺寸为50nm。哌啶加载的NPS抑制TNBC细胞生长和诱导细胞凋亡，同时施加正常成纤维细胞。结论：通过NPS将哌啶的细胞毒性浓度与NPS发育到TNBC细胞，具有改善的生物利用度和生物流体中的溶解度是可行的。

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