...
首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >3D Culture Represents Apoptosis Induced by Trastuzumab Better than 2D Monolayer Culture
【24h】

3D Culture Represents Apoptosis Induced by Trastuzumab Better than 2D Monolayer Culture

机译:3D培养物代表Trastuzumab优于2D单层培养的细胞凋亡

获取原文
获取原文并翻译 | 示例
           

摘要

Background: Our hypothesis was that three-dimensional (3D) culture better represents differential in vivo responses to trastuzumab between PIK3CA-wild-type (wt) and mutant (mt) cell lines than does two-dimensional (2D) culture. Materials and Methods: Apoptosis and cell signaling proteins were evaluated in response to trastuzumab with and without BKM120, a pan-phosphatidylinositol 3-kinase (PI3K) inhibitor, using western blot analysis of four breast cancer cell lines with human epidermal growth factor receptor 2 (HER2) amplification. Results: Increased expression of cleaved poly (ADP-ribose) polymerase (PARP) was observed only in 3D-cultured PIK3CA-wt lines in response to trastuzumab, but not in 2D-cultured PIK3CA-wt or PIK3CA-mt lines. Decrease in the ratio of phosphorylated (p-)AKT to AKT in response to trastuzumab was more profound in PIK3CA-wt cells than in PIK3CA-mt cells in 3D culture, while the difference between PIK3CA genotypes was less apparent in 2D culture. Treatment with BKM120 and trastuzumab resulted in a stronger increase in cleaved PARP than either treatment alone. Conclusion: 3D Culture appears to better represent trastuzumab-induced apoptosis and resistance to trastuzumab associated with PIK3CA mutation.
机译:背景:我们的假设是三维(3D)培养物更好地代表PIK3CA-野生型(WT)和突变体(MT)细胞系中的曲体反应的差异,而不是二维(2D)培养物。材料和方法:响应于Trastuzumab的凋亡和细胞信号传导蛋白,使用BkM120,泛磷脂酰肌醇3-激酶(PI3K)抑制剂,使用与人表皮生长因子受体2的四种乳腺癌细胞系的蛋白质印迹分析( HER2)扩增。结果:响应于曲妥珠单抗,但仅在3D培养的PIK3CA-WT系中观察到切割的聚(ADP-核糖)聚合酶(PARP)的表达增加,但不含2D培养的PIK3CA-WT或PIK3CA-MT线。在PIK3CA-WT细胞中,磷酸化(P-)akt至akt与曲妥珠单抗的比率降低,比在3D培养中的Pik3Ca-Mt细胞中更深刻,而Pik3Ca基因型之间的差异在2D培养物中不太明显。用BKM120和Trastuzumab治疗导致切割PARP的较强增加而不是单独治疗。结论:3D培养似乎更好地代表了曲妥珠单抗诱导的凋亡和抗性与PIK3CA突变相关的曲妥珠单抗。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号