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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >Lidocaine Stimulates the Function of Natural Killer Cells in Different Experimental Settings
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Lidocaine Stimulates the Function of Natural Killer Cells in Different Experimental Settings

机译：Lidocaine在不同实验设置中刺激天然杀伤细胞的功能

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Background: One of the functions of natural killer (NK) cells is to eliminate cancer cells. The cytolytic activity of NK cells is tightly regulated by inhibitory and activation receptors located in the surface membrane. Lidocaine stimulates the function of NK cells at clinically relevant concentrations. It remains unknown whether this effect of lidocaine has an impact on the expression of surface receptors of NK cells, can uniformly stimulate across different cancer cell lines, and enhances the function of cells obtained during oncological surgery. Materials and Methods: NK cells from healthy donors and 43 patients who had undergone surgery for cancer were isolated. The function of NK cells was measured by lactate dehydrogenase release assay. NK cells were incubated with clinically relevant concentrations of lidocaine. By flow cytometry, we determined the impact of lidocaine on the expression of galactosylgalactosylxylosylprotein3-beta-glucuronosytranferase 1, marker of cell maturation (CD57), killer cell lectin like receptor A, inhibitory (NKG2A) receptors and killer cell lectin like receptor D, activation (NKG2D) receptors of NK cells. Differences in expression at p<0.05 were considered statistically significant. Results: Lidocaine increased the expression of NKG2D receptors and stimulated the function of NK cells against ovarian, pancreatic and ovarian cancer cell lines. Lidocaine also increased the cytolytic activity of NK cells from patients who underwent oncological surgery, except for those who had orthopedic procedures. Conclusion: Lidocaine showed an important stimulatory activity on NK cells. Our findings suggest that lidocaine might be used perioperatively to minimize the impact of surgery on NK cells.
机译：背景：天然杀伤剂（NK）细胞的功能之一是消除癌细胞。 NK细胞的细胞分解活性通过位于表面膜中的抑制和活化受体紧密调节。利卡那e在临床相关浓度下刺激NK细胞的功能。它仍然未知LIDocaine的这种效果是否对NK细胞的表面受体的表达产生影响，可以均匀地刺激不同癌细胞系，并增强在肿瘤手术期间获得的细胞的功能。材料和方法：来自健康供体的NK细胞和43例，患有用于癌症手术的43名患者。通过乳酸脱氢酶释放测定法测量NK细胞的功能。将NK细胞与临床相关的利多卡因浓度孵育。通过流式细胞术，确定利多卡因的影响在半乳糖糖基甲基糖基蛋白蛋白1，细胞成熟的标记（CD57）的标志物，杀伤细胞凝集素，如受体A，抑制（NKG2A）受体和杀手细胞凝集素，如受体D，活化（NKG2D）NK细胞的受体。 P <0.05的表达的差异被认为是统计学意义。结果：利多卡因增加了NKG2D受体的表达，并刺激了NK细胞对卵巢，胰腺癌和卵巢癌细胞的功能。 Lidocaine还增加了从肿瘤手术的患者中增加了NK细胞的细胞溶解活性，除了具有骨科手术的人。结论：利多卡因显示NK细胞的重要刺激活性。我们的研究结果表明，Lidocaine可能会围手术用，以最大限度地减少手术对NK细胞的影响。

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