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首页> 外文期刊>Antimicrobial agents and chemotherapy. >The Arginine Deiminase Operon Is Responsible for a Fitness Trade-Off in Extended-Spectrum-beta-Lactamase-Producing Strains of Escherichia coli
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The Arginine Deiminase Operon Is Responsible for a Fitness Trade-Off in Extended-Spectrum-beta-Lactamase-Producing Strains of Escherichia coli

机译：精氨酸脱氨酶型术语负责在大肠杆菌的扩展β-内酰胺酶产生菌株中的健身权衡

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摘要

We previously identified an operon involved in an arginine deiminase (ADI) pathway (arc operon) on a CTX-M-producing plasmid from an 0102-ST405 strain of Escherichia coll. As the ADI pathway was shown to be involved in the virulence of various Gram-positive bacteria, we tested whether the ADI pathway could be involved in the epidemiological success of extended-spectrum-beta-lactamase (ESBL)-producing E. coli strains. We studied two collections of human E. coli isolated in France (n = 493) and England (n = 1,509) and show that the prevalence of the arc operon (i) is higher in ESBL-producing strains (12.1%) than in nonproducers (2.5%), (ii) is higher in CTX-M-producing strains (16%) than in other ESBL producers (3.5%), and (iii) increased over time in ESBL-producing strains from 0% before 2000 to 43.3% in 2011 to 2012. The arc operon, found in strains from various phylogenetic backgrounds, is carried by IncE plasmids (85%) or chromosomes (15%) in regions framed by numerous insertion sequences, indicating multiple arrivals. Competition experiments showed that the arc operon enhances fitness of the strain in vitro in lysogeny broth with arginine. In vivo competition experiments showed that the arc operon is advantageous for the strain in a mouse model of urinary tract infection (UTI), whereas it is a burden in a mouse model of intestinal colonization. In summary, we have identified a trait linked to CTX-M-producing strains that is responsible for a trade-off between two main E. coil lifestyles, UTI and gut commensalism. This trait alone cannot explain the wide spread of ESBLs in E. coli but merits epidemiological surveillance.
机译：我们之前鉴定了在从Escherichia Coll的0102-ST405菌株的CTX-M产生质粒上识别参与精氨酸脱氨酶（ADI）途径（ARC OVERON）的操纵子。随着ADI途径被涉及各种革兰氏阳性细菌的毒力，我们测试了ADI途径是否可以参与扩展光谱 - β-内酰胺酶（ESBL）的流行病学成功 - 发放大肠杆菌菌株。我们研究了在法国（n = 493）和英格兰（n = 1,509）中孤立的两种人类大肠杆菌的集合，并表明ESBL的菌株（12.1％）的Arc Operon（i）的患病率高于非产量（2.5％），（ii）在CTX-M产生菌株（16％）高于其他ESBL生产商（3.5％），（III）在ESBL的产量增加到2000年之前的0％至43.3 ％在2011年至2012年。在各种系统发育背景的菌株中发现的弧度操纵子由多种插入序列构成的区域中的Ince质粒（85％）或染色体（15％）携带，表明多个抵达。竞争实验表明，Arc Operon在具有精氨酸的溶酶体肉汤中的体外菌株的适应性。在体内竞争实验表明，弧度操纵子对泌尿道感染的小鼠模型中的菌株有利，而这是肠道殖民化小鼠模型的负担。总之，我们已经确定了与CTX-M产生菌株相关的特征，该菌株负责两种主要E.卷材生物，UTI和GUT共识之间的权衡。独自的这种特质无法解释E.Coli中的ESBLS广泛传播，但符合流行病学监督。

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  • 作者

    Billard-Pomares Typhaine; Clermont Olivier; Castellanos Miguel; Magdoud Fatma; Royer Guilhem; Condamine Benedicte; Fouteau Stephane; Barbo Valerie; Roche David; Cruveiller Stephane; Medigue Claudine; Pognard Dominique; Glodt Jeremy; Dion Sara; Rigal Odile; Picard Bertrand; Denamur Erick; Branger Catherine;

  • 作者单位

    Univ Paris 13 Sorbonne Paris Cite Univ Paris Diderot IAME UMR 1137 INSERM Paris France;

    Univ Paris 13 Sorbonne Paris Cite Univ Paris Diderot IAME UMR 1137 INSERM Paris France;

    Univ Paris 13 Sorbonne Paris Cite Univ Paris Diderot IAME UMR 1137 INSERM Paris France;

    Hop Avicenne AP HP Serv Microbiol Clin Bobigny France;

    Univ Paris 13 Sorbonne Paris Cite Univ Paris Diderot IAME UMR 1137 INSERM Paris France;

    Univ Paris 13 Sorbonne Paris Cite Univ Paris Diderot IAME UMR 1137 INSERM Paris France;

    Univ Paris Saclay Univ Evry CNRS CEA Inst Biol Francois Jacob Genoscope Genom Meta Evry France;

    Univ Paris Saclay Univ Evry CNRS CEA Inst Biol Francois Jacob Genoscope Genom Meta Evry France;

    Univ Evry Val dEssonne CEA CNRS Inst Genom Genoscope UMR 8030 Lab Anal Bioinforma Evry France;

    Univ Evry Val dEssonne CEA CNRS Inst Genom Genoscope UMR 8030 Lab Anal Bioinforma Evry France;

    Univ Paris Saclay Univ Evry CNRS CEA Inst Biol Francois Jacob Genoscope Genom Meta Evry France;

    Hop Louis Mourier AP HP Serv Microbiol Colombes France;

    Univ Paris 13 Sorbonne Paris Cite Univ Paris Diderot IAME UMR 1137 INSERM Paris France;

    Univ Paris 13 Sorbonne Paris Cite Univ Paris Diderot IAME UMR 1137 INSERM Paris France;

    Hop Robert Debre Serv Biochim Hormonol Paris France;

    Univ Paris 13 Sorbonne Paris Cite Univ Paris Diderot IAME UMR 1137 INSERM Paris France;

    Univ Paris 13 Sorbonne Paris Cite Univ Paris Diderot IAME UMR 1137 INSERM Paris France;

    Univ Paris 13 Sorbonne Paris Cite Univ Paris Diderot IAME UMR 1137 INSERM Paris France;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 治疗学;
  • 关键词

    Escherichia coli; plasmids;

    机译：大肠杆菌;质粒;

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