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首页> 外文期刊>Aquaculture >Comparative study on phenoloxidase activity of biofloc-reared pacific white shrimp Penaeus vannamei and Indian white shrimp Penaeus indicus on graded protein diet
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Comparative study on phenoloxidase activity of biofloc-reared pacific white shrimp Penaeus vannamei and Indian white shrimp Penaeus indicus on graded protein diet

机译：生物活植物脱脂太平洋虾苯甲酸盐酶活性的比较研究vannamei和印度白虾Penaeus indums对分级蛋白饮食

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摘要

Shrimp aquaculture demands technological advancement that boosts production and environmental sustainability. This study evaluated and compared the growth performance and innate immunity of two species of shrimp (Pacific White shrimp Penaeus vannamei and India white shrimp Penaeus indicus) reared under biofloc technology with different dietary protein levels (Low-25%, Medium-30% and High-35%). As prophenoloxidase (proPO) activity is an important parameter in immune response of shrimp, we focused on the dynamics of prophenoloxidase induced by three different activators (Sodium dodecyle sulfate, (SDS, Trypsin & Laminarin). At the end of the experiment, the effect of activators on proPO activity in serum, plasma and hemocyte lysate (HLS) was evaluated in both the species under conventional and biofloc systems with graded proteins. This experiment lasted for a month as nursery rearing followed by 60 days of grow-out culture. There was no significant difference (p < 0.05) in growth performances of both the species reared under biofloc, whereas control group exhibited lower growth. Significantly higher average body weight (ABW), survival and specific growth rate (SGR) were observed in medium protein groups reared under biofloc than the other biofloc groups and control. The medium protein groups also showed significantly lower (p < 0.01) TAN, NO2-N, and NO3-N levels compared to other treatments and the control exhibited considerably higher TAN, NO2-N and NO3-N levels. The proPO activity in serum of both the shrimps were found to be significantly higher (p < 0.05) than the control in which highest proPO activity was observed in SDS activated groups irrespective of the graded protein levels. A similar trend was observed in the plasma of P. indicus in which SDS activated group fed with high protein level showed highest proPO activity, whereas in plasma of P. vannamei trypsin activated groups fed with low protein level showed higher proPO activity than SDS and laminarin activated groups. The HLS of P. vannamei showed highest proPO functioning in trypsin activated group fed with high protein level, whereas in P. indicus, the highest proPO activity was observed in SDS activated group fed with high protein diet. Though in many cases, the groups fed with high protein diet showed highest proPO activity, the low protein and the medium protein groups also exhibited upregulation in the immune response parameter reared under biofloc irrespective of the species. SOD activity of the hemocytes were influenced by different dietary protein levels and better inhibition was observed in HP when compared to control and other treated groups. Thus the native non-SPF Indian white shrimp P. indicus has comparable potential as the SPF Pacific white shrimp P. vannamei in terms of innate immunity, immune response, and growth when reared under biofloc conditions.
机译：虾水产养殖要求推动生产和环境可持续性的技术进步。该研究评估并比较了两种虾（太平洋白虾Penaeus Vannamei和印度白虾Penaeus indumus）的生长性能和先天免疫力，在生物氟化技术中饲养，不同的膳食蛋白水平（低25％，中 - 30％和高-35％）。作为丙戊烷氧基酶（PROPO）活性是虾的免疫应答的重要参数，我们专注于三种不同活化剂（十二型硫酸钠钠，（SDS，胰蛋白酶和层状）诱导的前甲氧化酶的动态。在实验结束时，效果在具有分级蛋白的常规和生物氟氯化体系下的种类中评估了血清，血浆和血细胞裂解物（HLS）中的ProPo活性的活化剂。这项实验持续了一个月作为幼儿园饲养，然后进行60天的成长文化。那里在生物氟氯丙酸下饲养的各种物种的生长性能下没有显着差异（P <0.05），而对照组表现出较低的生长。在中等蛋白质组中观察到较高的平均体重（ABW），存活率和特异性生长速率（SGR）与其他生物氟化群组和对照组饲养。中等蛋白质组也显示出与OTH相比显着降低（P <0.01）TAN，NO2-N和NO3-N水平ER治疗和对照表现出相当高的TAN，NO2-N和NO 3-N水平。发现虾血清的ProPo活性被发现明显高（P <0.05），而不是在SDS活化组中观察到最高PROP活性的对照，而不论分级蛋白质水平如何。在P的血浆中观察到类似的趋势，其中具有高蛋白质水平的SDS激活组显示出最高的ProPo活性，而在vannamei胰蛋白酶激活的血浆中血浆，饲喂低蛋白质水平的激活组比SDS和层状蛋白酶更高激活的组。 P.Vannamei的HLS显示出具有高蛋白质水平的胰蛋白酶活化组中的最高ProPo功能，而在P. indecus中，在具有高蛋白质饮食的SDS活性组中观察到最高的ProPo活性。虽然在许多情况下，用高蛋白质饮食喂养的群体显示出最高的ProPo活性，低蛋白质和中蛋白基团也在生物氟普洛夫饲养的免疫应答参数中表现出上调，而不管物种如何。与对照和其他处理的基团相比，血细胞的SOD活性受到不同膳食蛋白水平的影响，并且在HP中观察到更好的抑制。因此，本土非SPF印度白虾P. indicus在先天免疫，免疫应答和在生物氟化术条件下饲养时，indipus的潜在与SPF太平洋白虾P.Vannamei具有相当的潜力。

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  • 来源
    《Aquaculture》 |2020年第2020期|共10页
  • 作者

    Panigrahi A.; Sivakumar M. R.; Sundaram M.; Saravanan A.; Das R. R.; Katneni V. K.; Ambasankar K.; Dayal J. Syama; Gopikrishna G.;

  • 作者单位

    ICAR Cent Inst Brackishwater Aquaculture Crustacean Culture Div 75 Santhome High Rd Chennai 600028 Tamil Nadu India;

    ICAR Cent Inst Brackishwater Aquaculture Crustacean Culture Div 75 Santhome High Rd Chennai 600028 Tamil Nadu India;

    ICAR Cent Inst Brackishwater Aquaculture Crustacean Culture Div 75 Santhome High Rd Chennai 600028 Tamil Nadu India;

    ICAR Cent Inst Brackishwater Aquaculture Crustacean Culture Div 75 Santhome High Rd Chennai 600028 Tamil Nadu India;

    ICAR Cent Inst Brackishwater Aquaculture Crustacean Culture Div 75 Santhome High Rd Chennai 600028 Tamil Nadu India;

    ICAR Cent Inst Brackishwater Aquaculture Crustacean Culture Div 75 Santhome High Rd Chennai 600028 Tamil Nadu India;

    ICAR Cent Inst Brackishwater Aquaculture Crustacean Culture Div 75 Santhome High Rd Chennai 600028 Tamil Nadu India;

    ICAR Cent Inst Brackishwater Aquaculture Crustacean Culture Div 75 Santhome High Rd Chennai 600028 Tamil Nadu India;

    ICAR Cent Inst Brackishwater Aquaculture Crustacean Culture Div 75 Santhome High Rd Chennai 600028 Tamil Nadu India;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 水产、渔业;
  • 关键词

    SPF; P. vannamei; P. indicus; Biofloc; Phenoloxidase; Immunity;

    机译：SPF;p。 Vannamei;p。 indecus;生物氟化酶;酚氧基酶;免疫力;

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